Nardosinone enhances nerve growth factor-induced neurite outgrowth in a mitogen-activated protein kinase- and protein kinase C-dependent manner in PC12D cells
Nardosinone enhances nerve growth factor-induced neurite outgrowth in a mitogen-activated protein kinase- and protein kinase C-dependent manner in PC12D cells
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DOI：
10.1254/jphs.93.122
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发表时间：
2003-09-01
影响因子：
3.5
通讯作者：
Ohizumi, Y
中科院分区：
文献类型：
--
作者：
Li, P;Yamakuni, T;Ohizumi, Y
研究了纳多西酮促进神经生长因子（NGF, 2 ng/ml）诱导PC12D细胞神经突生长的机制。结果表明，nardosinone对ngf诱导的神经突生长的增强依赖于丝裂原活化蛋白（MAP）激酶，但不伴有ngf诱导的MAP激酶磷酸化的增加。此外，蛋白激酶C （PKC）抑制剂GF109203X可以消除ngf诱导的神经突生长的增强。这些结果表明，nardosinone对NGF诱导的PC12D细胞神经突生长的增强涉及激活NGF与PKC的MAP激酶依赖级联的下游步骤。
The mechanism to enhance nerve growth factor (NGF, 2 ng/ml)-induced neurite outgrowth from PC12D cells by nardosinone isolated from Nardostachys chinensis was examined. It was shown that the potentiation of the NGF-induced neurite outgrowth by nardosinone was mitogen-activated protein (MAP) kinase-dependent, but was not accompanied by stimulation of NGF-induced increase in MAP kinase phosphorylation. Furthermore, this augmentation of NGF-induced neurite outgrowth was abolished by GF109203X, a protein kinase C (PKC) inhibitor. These results suggest that the enhancement of NGF-induced neurite outgrowth from PC12D cells by nardosinone involves activation of a down-stream step of the MAP kinase-dependent cascade of NGF coupled with PKC.