IMMUNOLABELING OF THE PRESYNAPTIC MEMBRANE OF TORPEDO ELECTRIC ORGAN NERVE-TERMINALS WITH AN ANTISERUM TOWARDS THE ACETYLCHOLINE-RELEASING PROTEIN MEDIATOPHORE
IMMUNOLABELING OF THE PRESYNAPTIC MEMBRANE OF TORPEDO ELECTRIC ORGAN NERVE-TERMINALS WITH AN ANTISERUM TOWARDS THE ACETYLCHOLINE-RELEASING PROTEIN MEDIATOPHORE
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DOI：
10.1016/0248-4900(93)90125-x
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发表时间：
1993-01-01
影响因子：
2.7
通讯作者：
LESBATS, B
中科院分区：
文献类型：
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作者：
BROCHIER, G;ISRAEL, M;LESBATS, B
介质载体是一种从鱼雷电器官中纯化出来的神经末梢膜蛋白，其在钙作用下具有转运乙酰胆碱的能力。一种能够免疫沉淀介质团活性的抗血清被用来研究该蛋白的亚细胞分布。突触前膜表现出强烈和不连续的免疫金标记，特别是在被认为释放ACh的活性区。在突触体膜的免疫印迹上识别出两种抗原：媒质载体的15-kDa亚基和具有广泛非神经元分布的14-kDa膜蛋白。从血清中纯化的抗体在天然介质上对15kda抗原具有单特异性，仍然具有较高的突触前膜定位标记。此外，在活性区存在少量14kda蛋白位点。介于突触前膜和突触后弓之间的雪旺细胞指也显示出14-kDa抗原，这提出了来自雪旺细胞的14-kDa蛋白可能与中介细胞相互作用的问题。
Mediatophore is a nerve terminal membrane protein purified from Torpedo electric organ on its ability to translocate acetylcholine upon calcium action. An antiserum able to immunoprecipitate mediatophore activity was used to study the subcellular distribution of this protein. The presynaptic membrane exhibited a strong and discontinuous immunogold labelling, especially at the active zone where ACh is thought to be released. Two antigens were recognized on immunoblots of synaptosomal membranes: the 15-kDa subunit of mediatophore and a 14-kDa membrane protein that has a wide non-neuronal distribution. Antibodies purified from the serum on native mediatophore and monospecific towards the 15-kDa antigen still gave a high presynaptic membrane localized labelling. In addition, a few 14-kDa protein sites were present at the active zone. The Schwann cell finger interposed between the presynaptic membrane and the postsynaptic arch also exhibited the 14-kDa antigen raising the question of a possible interaction of mediatophore with the 14-kDa protein originating from the Schwann cell.