Impact of Expressing Cells on Glycosylation and Glycan of the SARS-CoV-2 Spike Glycoprotein.
Impact of Expressing Cells on Glycosylation and Glycan of the SARS-CoV-2 Spike Glycoprotein.
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DOI：
10.1021/acsomega.1c01785
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发表时间：
2021-06-22
期刊：
影响因子：
4.1
通讯作者：
Yang S
中科院分区：
文献类型：
--
作者：
Wang Y;Wu Z;Hu W;Hao P;Yang S
The spike glycoprotein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the first point of contact for the virus to recognize and bind to host receptors, is the focus of biomedical research seeking to effectively prevent and treat coronavirus disease (COVID-19). The mass production of spike glycoproteins is usually carried out in different cell systems. Studies have been shown that different expression cell systems alter protein glycosylation of hemagglutinin and neuraminidase in the influenza virus. However, it is not clear whether the cellular system affects the spike protein glycosylation. In this work, we investigated the effect of an expression system on the glycosylation of the spike glycoprotein and its receptor-binding domain. We found that there are significant differences in the glycosylation and glycans attached at each glycosite of the spike glycoprotein obtained from different expression cells. Since glycosylation at the binding site and adjacent amino acids affects the interaction between the spike glycoprotein and the host cell receptor, we recognize that caution should be taken when selecting an expression system to develop inhibitors, antibodies, and vaccines.
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影响因子：
5.4
作者：
Heurich, Adeline;Hofmann-Winkler, Heike;Poehlmann, Stefan
通讯作者：
Poehlmann, Stefan
对昆虫和人类细胞中产生的 SARS-CoV-2 刺突蛋白进行位点特异性 O 糖基化分析。
DOI：
10.3390/v13040551
发表时间：
2021-03-25
期刊：
Viruses
影响因子：
--
作者：
Bagdonaite I;Thompson AJ;Wang X;Søgaard M;Fougeroux C;Frank M;Diedrich JK;Yates JR 3rd;Salanti A;Vakhrushev SY;Paulson JC;Wandall HH
通讯作者：
Wandall HH
影响因子：
4.8
作者：
May, Carolyn;Ji, Suena;Ten Hagen, Kelly G.
通讯作者：
Ten Hagen, Kelly G.
影响因子：
4.3
作者：
Kong, Yun;Joshi, Hiren J.;Clausen, Henrik
通讯作者：
Clausen, Henrik
影响因子：
11.4
作者：
Lukassen, Soeren;Chua, Robert Lorenz;Eils, Roland
通讯作者：
Eils, Roland