Genetic characterization of six species of diplozoids (Monogenea; Diplozoidae)
Genetic characterization of six species of diplozoids (Monogenea; Diplozoidae)
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DOI：
10.1017/s0031182001008617
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发表时间：
2001-11-01
期刊：
影响因子：
2.4
通讯作者：
Cunningham, CO
中科院分区：
文献类型：
--
作者：
Matejusová, I;Koubková, B;Cunningham, CO
利用PCR技术扩增了日本真二倍体虫（Eudiplozoon nipponicum）、布氏副二倍体虫（Paradiplozoon bliccae）、人二倍体虫（P.homoion）、梅根二倍体虫（P.megan）、巴甫洛夫斯基二倍体虫（P.pavlovskii）和沙皮二倍体虫（P.sapae）6种二倍体虫核糖体RNA基因阵列的第二个内转录间隔区（ITS 2），并进行了序列测定。这些序列清楚地表明在物种水平上的歧视，并确认形态鉴定确定的物种的有效性。ITS2序列未发现种内变异。不同寄主群体间的同源性分析结果表明，同源性分析结果与寄主群体间的同源性分析结果一致。PCR产物的长度可以区分E.来自副二倍体虫种的nipponicum用酶AluI、HaeIII和HinfI消化扩增的ITS 2片段，为物种鉴定提供了有用的遗传标记。二倍体之间的亲缘关系再次证明E. nipponicum是遗传上最不同的物种，而P.bliccae和P.sapae是最接近的物种。这是第一个分子分类学研究这些有趣的寄生虫，并证明了这些方法的效用，解决系统学问题。
The second internal transcribed spacer (ITS2) of the ribosomal RNA gene array of 6 species of diplozoids; Eudiplozoon nipponicum, Paradiplozoon bliccae, P. homoion, P. megan, P. pavlovskii and P. sapae, was amplified by PCR and sequenced. These sequences clearly demonstrate discrimination at the species level and confirm the validity of species determined by morphological identification. No intraspecific variation was found in the ITS2 sequences. There were no differences in the ITS2 sequences of P. homoion from populations parasitizing different host species. The length of the PCR product allowed discrimination of E. nipponicum from the Paradiplozoon species. Digestion of the amplified ITS2 fragment with enzymes AluI, HaeIII and HinfI provided useful genetic markers for species identification. The genetic relationships between diplozoids again demonstrated that E. nipponicum was the most genetically distinct species, whereas P. bliccae and P. sapae were the species most closely related. This represents the first molecular taxonomic study of these interesting parasites and demonstrates the utility of these methods for addressing questions of systematics.