Zebrafish embryology and cartilage staining protocols for high school students.
Zebrafish embryology and cartilage staining protocols for high school students.
复制标题
高中生斑马鱼胚胎学和软骨染色方案。
作者：
Farida Emran;Jacqueline M. Brooks;Steven Zimmerman;Susan L. Johnson;R. Lue
哈佛大学生命科学-霍华德休斯医学研究所外展项目支持高中科学教育，为学生和他们的老师提供一个校园项目，让他们参与调查和动手实验。外展计划最近设计并成功地推出了斑马鱼胚胎学方案，我们在这里展示。该方案的主要目的是向学生介绍斑马鱼作为一种模型研究生物，并为学生提供胚胎学研究中使用的当前技术的直接经验。实验的内容旨在产生关于胚胎学、遗传学、受精、自然选择和动物适应的讨论。该方案使用最少的试剂，以时间高效的方式产生可靠的结果。本文提出的方案包括三个部分：观察不同发育阶段的活斑马鱼幼虫，斑马鱼幼虫的软骨染色，以及包括鉴定两种斑马鱼突变体（珍珠和chokh）的突变体狩猎。在这里，我们描述了该方案，显示了每个部分获得的结果，并提出了不同实验室设置的可能替代方案。
The Life Sciences-Howard Hughes Medical Institute Outreach Program at Harvard University supports high school science education by offering an on-campus program for students and their teachers to participate in investigative, hands-on laboratory sessions. The outreach program has recently designed and launched a successful zebrafish embryology protocol that we present here. The main objectives of this protocol are to introduce students to zebrafish as a model research organism and to provide students with direct experience with current techniques used in embryological research. The content of the lab is designed to generate discussions on embryology, genetics, fertilization, natural selection, and animal adaptation. The protocol produces reliable results in a time-efficient manner using a minimum of reagents. The protocol presented here consists of three sections: observations of live zebrafish larvae at different developmental stages, cartilage staining of zebrafish larvae, and a mutant hunt involving identification of two zebrafish mutants (nacre and chokh). Here, we describe the protocol, show the results obtained for each section, and suggest possible alternatives for different lab settings.
影响因子：
2.7
作者：
Du, SJ;Frenkel, V;Zohar, Y
通讯作者：
Zohar, Y
珍珠层编码一种与斑马鱼小眼球相关的蛋白质，该蛋白质调节神经嵴衍生的色素细胞的命运。
DOI：
10.1242/dev.126.17.3757
发表时间：
1999
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Lister,JA;Robertson,CP;Lepage,T;Johnson,SL;Raible,DW
通讯作者：
Raible,DW