Characterization of the complete genome of a novel citrivirus infecting Actinidia chinensis
Characterization of the complete genome of a novel citrivirus infecting Actinidia chinensis
复制标题
DOI：
10.1007/s00705-013-1654-2
复制
发表时间：
2013-08-01
影响因子：
2.7
通讯作者：
Pearson, Michael N.
中科院分区：
文献类型：
--
作者：
Chavan, Ramesh R.;Blouin, Arnaud G.;Pearson, Michael N.
猕猴桃（Actinidia spp.）被鉴定为β-反曲病毒科的成员。它通过机械方式传播到草本指示植物本氏烟草（Nicotianabenthamiana）、烟草（N. clevelandii，拟野牡丹N. nanososa和N. [咒语]完整的基因组由三个ORF和一个3 'poly（A）尾组成。全基因组的系统发育分析表明，它是一个新的成员属Citrivirus（家庭Betaflexiviridae）。其核苷酸序列与柑桔叶斑病毒（CLBV）的核苷酸序列相差约26%。其运动蛋白（ORF 2）和外壳蛋白（ORF 3）与CLBV的氨基酸序列同源性分别为95- 96%和90- 92%。复制酶多聚蛋白（ORF 1）与已发表的CLBV序列明显不同，具有78- 79%的氨基酸序列同一性，而5' UTR和3' UTR与CLBV的差异分别为28%和29%。序列差异表明，来自猕猴桃的柑橘病毒是CLBV的分歧株或一个新的柑橘病毒种的成员。
A ssRNA virus from kiwifruit (Actinidia spp.) was identified as a member of the family Betaflexiviridae. It was mechanically transmitted to the herbaceous indicators Nicotiana benthamiana, N. clevelandii, N. glutinosa and N. occidentalis. The complete genome was comprised of three ORFs and a 3'poly (A) tail. Phylogenetic analysis of the entire genome indicated it was a novel member of the genus Citrivirus (family Betaflexiviridae). The complete nucleotide sequence differed from that of citrus leaf blotch virus (CLBV) by similar to 26 %. The movement protein (ORF2) and coat protein (ORF3) shared 95-96 % and 90-92 % amino acid sequence identity, respectively, with CLBV. The replicase polyprotein (ORF1) was distinctly different from published CLBV sequences, with 78-79 % amino acid sequence identity, while the 5' UTR and 3' UTR differed from CLBV by 28 % and 29 %, respectively. The sequence differences indicate that the citrivirus from Actinidia is either a divergent strain of CLBV or a member of a new citrivirus species.