Phase transition properties of 1,2- and 1,3-diacylphosphatidylethanolamines with modified head groups.
Phase transition properties of 1,2- and 1,3-diacylphosphatidylethanolamines with modified head groups.
复制标题
具有修饰头基的 1,2- 和 1,3-二酰基磷脂酰乙醇胺的相变特性。
DOI：
10.1021/bi00336a045
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Dalziel,AW
中科院分区：
文献类型：
--
作者：
Chowdhry,BZ;Dalziel,AW
耶鲁大学化学系，纽黑文，康涅狄格州 06511 1984 年 11 月 20 日收到摘要：“非水合”（即未加热至高于室温或高于完全水合脂质的主要相变温度，以较低者为准的脂质悬浮液）和水合 1.2 (a)-和 l, 3 的稀水悬浮液的相变特性头基修饰的 (/3)-二棕榈酰磷脂酰乙醇胺已通过高灵敏度差示扫描量热法以 0.1 K min™ 1 的扫描速率进行测定。在 1、2 和 1, 3 系列中，磷酸乙醇胺部分的头基修饰包括 TV-甲基、TV/V-二甲基和 TV。 AyV-三甲基（磷酸胆碱）。在1, 2系列中，额外的修饰有二硝基苯基、三硝基苯基、7V-(二硝基苯基)氨基己酰基、N-(三硝基苯基)氨基己酰基和7V-4-硝基-2,1,3-苯并恶二唑。本研究还包括 1, 2-二十六烷基磷脂酰乙醇胺和相应的 N-甲基取代脂质。一般来说，头基取代基体积的增加导致转变温度不断降低，水合脂质中最极端的情况是由N-(二硝基-苯基)氨基己酰基取代及其1、2系列中的三硝基苯基类似物产生的45°C降低。量热跃迁焓的变化没有明显的简单趋势。
Department of Chemistry, Yale University, New Haven, Connecticut 06511 Received November 20, 1984 abstract: The phase transition properties of dilute aqueous suspensions of “nonhydrated”(ie, lipid suspensions which had not been heated above room temperature or above the main phase transition tem-perature of the fully hydrated lipid, whichever was lower) and hydrated 1.2 (a)-and l, 3 (/3)-dipalmitoyl-phosphatidylethanolamines with modified head groups have been determined by high-sensitivity differential scanning calorimetry at a scan rate of 0.1 K min™ 1. In both the 1, 2 and 1, 3 series, the head-group modifications of the phosphoethanolaminemoiety included TV-methyl, TVJV-dimethyl, and TV. AyV-trimethyl (phosphocholine). In the 1, 2 series, additional modifications were dinitrophenyl, trinitrophenyl, 7V-(dinitrophenyl) aminocaproyl, N-(trinitrophenyl) aminocaproyl, and 7V-4-nitro-2, 1, 3-benzoxadiazole. Also included in this study were 1, 2-dihexadecylphosphatidylethanolamine and the corresponding N-methyl-substituted lipid. In general, increasing bulkiness of the head-group substituent caused increasing lowering of the transition temperature, the most extreme cases among the hydrated lipids being the 45 C lowering produced by the iV-(dinitro-phenyl) aminocaproyl substitution and its trinitrophenyl analogue in the 1, 2 series. No simple trend is evident in thechanges produced in the calorimetric enthalpy of transitions.