Advanced In Vitro Assay System to Measure Phosphatidylserine and Phosphatidylethanolamine Transport at ER/Mitochondria Interface
Advanced In Vitro Assay System to Measure Phosphatidylserine and Phosphatidylethanolamine Transport at ER/Mitochondria Interface
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先进的体外测定系统可测量 ER/线粒体界面处的磷脂酰丝氨酸和磷脂酰乙醇胺转运
DOI：
10.1007/978-1-4939-9136-5_6
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Endo Toshiya
中科院分区：
文献类型：
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作者：
Tamura Yasushi;Kojima Rieko;Endo Toshiya
先前的许多研究表明，磷脂分子在内质网和线粒体膜之间往返，而非囊状磷脂转运的分子基础仍不清楚。在本章中，我们描述了一种优化的方法，该方法使用从酵母细胞分离的膜组分直接分析内质网和线粒体之间的磷脂运输。通过这种分析，我们不仅能够评估er到线粒体的运输，还能够同时评估线粒体到er的运输。我们相信该检测系统可以加速细胞器间磷脂转运的研究。
A number of previous studies have shown that phospholipid molecules come and go between the endoplasmic reticulum (ER) and mitochondrial membranes while the molecular basis of non-vesicular phospholipid transport is still not understood well. In this chapter, we describe an optimized method that uses membrane fractions isolated from yeast cells to directly analyze phospholipid transport between the ER and mitochondria. With this assay, we are able to assess not only the ER-to-mitochondria but also mitochondria-to-ER transports at the same time. We believe that this assay system can accelerate the research on inter-organelle phospholipid trafficking.