USE OF BOVINE-MILK CONCENTRATE CONTAINING ANTIBODY TO ROTAVIRUS TO TREAT ROTAVIRUS GASTROENTERITIS IN INFANTS
USE OF BOVINE-MILK CONCENTRATE CONTAINING ANTIBODY TO ROTAVIRUS TO TREAT ROTAVIRUS GASTROENTERITIS IN INFANTS
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DOI：
10.1093/infdis/156.1.158
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发表时间：
1987-07-01
影响因子：
6.4
通讯作者：
WERCHAU, H
中科院分区：
文献类型：
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作者：
HILPERT, H;BRUSSOW, H;WERCHAU, H
使用含有从轮状病毒超免疫奶牛制备的乳汁免疫球蛋白的浓缩物（中和滴度，10%溶液为1：6，000）治疗因急性轮状病毒胃肠炎住院的婴儿，导致病毒排泄持续时间显著缩短（P = 0.008）。接受治疗婴儿的粪便样本显示，47%的病例中存在牛乳免疫球蛋白，43%的病例中存在中和活性（平均中和滴度，1：48）;对照婴儿的粪便样本仅3%的病例中显示中和活性（中和滴度，< 1：20）。粪便提取物的免疫电泳显示片段A，F（ab“）2或Fab的牛类似物，作为免疫球蛋白体外和体内消化的主要产物。25名婴儿中有19名出现中和活性，与病毒排泄停止相关。仅粪便中具有高中和活性的浓缩物治疗婴儿显示出病毒排泄持续时间的统计学显著性缩短;中和活性低的浓缩物治疗婴儿的该持续时间与对照组相当。
The use of a concentrate containing milk immunoglobulins prepared from rotavirus-hyperimmunized cows (neutralization titer, 1:6,000 for a 10% solution) to treat infants hospitalized for acute rotavirus gastroenteritis resulted in a significant (P = .008) reduction in the duration of excretion of virus. Stool samples from treated infants showed the presence of bovine milk immunoglobulins in 47% of cases and of neutralizing activity in 43% (mean neutralization titer, 1:48); stool samples from control infants showed neutralizing activities in only 3% of cases (neutralization titers, < 1:20). Immunoelectrophoresis of stool extracts revealed fragment A, a bovine analogue of F(ab'')2 or Fab, as the major product of in vitro and in vivo digestion of the immunoglobulins. Cessation of excretion of virus correlated with the appearance of neutralizing activities in 19 of 25 infants. Only concentrate-treated infants with high neutralizing activity in stools showed a statistically significant reduction in duration of excretion of virus; this duration in concentrate-treated infants with low neutralizing activity was comparable with controls.