Effect of tunicamycin on IgM, IgA, and IgG secretion by mouse plasmacytoma cells.
Effect of tunicamycin on IgM, IgA, and IgG secretion by mouse plasmacytoma cells.
复制标题
衣霉素对小鼠浆细胞瘤细胞 IgM、IgA 和 IgG 分泌的影响。
DOI：
10.4049/jimmunol.121.3.990
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发表时间：
1978
影响因子：
4.4
通讯作者：
S. Kornfeld
中科院分区：
文献类型：
--
作者：
S. Hickman;S. Kornfeld
衣霉素是一种通过阻断N-乙酰葡糖胺-脂质中间体的形成来防止糖蛋白糖基化的抗生素，用于研究糖基化对于产生不同类别免疫球蛋白的小鼠浆细胞瘤系分泌免疫球蛋白的重要性。生物合成标记的分泌和细胞内免疫球蛋白的免疫沉淀测定。0.5 μ g/ml的衣霉素对MOPC 104 E浆细胞的IgM分泌产生81%的抑制，而不显著影响细胞内IgM合成的初始速率。未证实非糖基化IgM的细胞内降解增加。衣霉素也产生了64%的平均抑制伊加分泌的几个小鼠IgA分泌型浆细胞瘤株。相比之下，尽管抑制D-[14 C]葡糖胺掺入新合成的IgG，衣霉素仅产生28%的IgG分泌平均抑制，这仅略高于变体MOPC 315浆细胞瘤对正常非糖基化IgG 2轻链分泌的非特异性抑制。这些数据表明，衣霉素产生的免疫球蛋白分泌的抑制程度取决于浆细胞产生的免疫球蛋白类别。
Tunicamycin, an antibiotic that prevents glycosylation of glycoproteins by blocking the formation of N-acetylglucosamine-lipid intermediates, was used to study the importance of glycosylation for the secretion of immunoglobulins by mouse plasmacytoma lines that produce immunoglobulins of different classes. Biosynthetically labeled secreted and intracellular immunoglobulins were measured by immunoprecipitation assays. Tunicamycin, at a concentration of 0.5 mug/ml produced an 81% inhibition of IgM secretion by MOPC 104E plasma cells without significantly affecting the initial rate of synthesis of intracellular IgM. No increase in the intracellular degradation of nonglycosylated IgM could be demonstrated. Tunicamycin also produced a 64% average inhibition of IgA secretion by several mouse IgA-secreting plasmacytoma lines. In contrast, despite inhibiting the incorporation of D-[14C] glucosamine into newly synthesized IgG, tunicamycin only produced a 28% average inhibition of IgG secretion, which was only slightly more than the nonspecific inhibition of secretion of the normally nonglycosylated lambda2 light chains by variant MOPC 315 plasmacytomas. These data indicate that the extent of inhibition of immunoglobulin secretion produced by tunicamycin depends on the immunoglobulin class produced by the plasma cell.