cj0371: A Novel Virulence-Associated Gene of Campylobacter jejuni.
cj0371: A Novel Virulence-Associated Gene of Campylobacter jejuni.
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cj0371：空肠弯曲杆菌的新型毒力相关基因
DOI：
10.3389/fmicb.2016.01094
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发表时间：
2016
影响因子：
5.2
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Du X;Wang N;Ren F;Tang H;Jiao X;Huang J
空肠弯曲杆菌是世界范围内人类细菌性腹泻的主要原因。其发病机制尚不清楚。Cj0371是通过免疫筛选发现的一个新基因。此前还没有关于它的功能的报道。在这项研究中，我们构建了该基因在空肠弯曲菌中的插入突变体和补体，并检测了毒力的变化。我们观察到突变体cj0371表现出明显的侵袭和定植能力。我们还研究了cj0371在运动性、趋化性和生长动力学中的作用，以进一步研究其功能。我们发现，突变体cj0371表现出超动力，趋化性增强，生长动力学增强。此外，我们还利用荧光显微镜将Cj0371蛋白定位于空肠弯曲菌的两极。我们提供的数据显著地证明了我们的假设，即cj0371是一个新的毒力相关基因，并通过趋化性的影响在空肠弯曲菌的致病性中发挥负面作用。
Campylobacter jejuni is the major cause of human bacterial diarrhea worldwide. Its pathogenic mechanism remains poorly understood. cj0371 is a novel gene that was uncovered using immunoscreening. There have been no previous reports regarding its function. In this study, we constructed an insertion mutant and complement of this gene in C. jejuni and examined changes in virulence. We observed that the cj0371 mutant showed significantly increased invasion and colonization ability. We also investigated the role of cj0371 in motility, chemotaxis, and growth kinetics to further study its function. We found that the cj0371 mutant displays hypermotility, enhanced chemotaxis, and enhanced growth kinetics. In addition, we localized the Cj0371 protein at the poles of C. jejuni by fluorescence microscopy. We present data that collectively significantly proves our hypothesis that cj0371 is a new virulence-associated gene and through the influence of chemotaxis plays a negative role in C. jejuni pathogenicity.