Nicotine synthesis in excised tobacco roots
Nicotine synthesis in excised tobacco roots
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DOI：
10.2307/2437647
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发表时间：
1942-12-01
影响因子：
3
通讯作者：
Dawson, RF
中科院分区：
文献类型：
--
作者：
Dawson, RF
切下的烟草 (Nicotiana tabacum) 变种的根。土耳其在含有矿物质营养素、蔗糖和酵母提取物的培养基中进行无菌培养。尼古丁以二苦酸盐形式从根部和废培养液中分离出来。在 8 个月的时间里，连续 6 次传代，生长和尼古丁的产生都是连续的。在任何一次传代过程中产生的大部分尼古丁都被根部排泄到外部介质中并在那里积累。这是器官培养过程中尼古丁合成的首次记录，并完成了尼古丁合成机制在烟草根中定位的证明。
Excised roots of Nicotiana tabacum var. Turkish were grown in sterile culture in a medium containing mineral nutrients, sucrose and yeast extract. Nicotine was isolated as the dipicrate from the roots and from the spent culture fluids. Growth and nicotine production were continuous throughout 6 successive passages over a period of 8 months. The bulk of the nicotine produced during any one passage was excreted by the roots into the external medium where it accumulated. This constitutes the first record of nicotine synthesis during growth in an organ culture and completes the proof for the localization of the nicotine synthetic mechanism in the tobacco root.