INVIVO, CAMP STIMULATES GROWTH AND MORPHOGENESIS OF MOUSE MAMMARY DUCTS
INVIVO, CAMP STIMULATES GROWTH AND MORPHOGENESIS OF MOUSE MAMMARY DUCTS
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DOI：
10.1073/pnas.81.15.4950
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
DANIEL, CW
中科院分区：
文献类型：
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作者：
SILBERSTEIN, GB;STRICKLAND, P;DANIEL, CW
在培养中，已知cAMP对乳腺细胞和其他几种上皮细胞有促有丝分裂作用，但在体内类似作用的证据仅是相关的。使用塑料植入物使霍乱毒素和其他cAMP活性剂缓慢释放到卵巢切除小鼠的乳腺局部区域。植入物周围细胞内cAMP水平升高促进了蓬勃生长和正常的导管形态发生，而远处部位不受影响。cAMP的局部作用包括恢复正常的导管口径，形成新的端芽和重新启动上皮细胞和周围基质中的DNA合成。因此，cAMP在该组织中既是促有丝分裂因子又是形态发生因子。
In culture, cAMP is known to be mitogenic for mammary cells and several other epithelia, but evidence for a similar role in vivo has been only correlative. Plastic implants were used to cause slow release of cholera toxin and other cAMP-active agents to local areas of mammary glands in ovariectomized mice. Elevated levels of intracellular cAMP around the implants promoted vigorous growth and normal ductual morphogenesis, while distant sites were unaffected. Local effects of cAMP included restoration of normal ductal caliber, formation of new end buds and reinitiation of DNA synthesis in both epithelium and surrounding stroma. Thus, cAMP is both a mitogenic and a morphogenetic factor in this tissue.