Two‐dimensional protein database of human pancreas
Two‐dimensional protein database of human pancreas
复制标题
人胰腺二维蛋白质数据库
DOI：
10.1002/elps.200305683
复制
发表时间：
2004
期刊：
影响因子：
2.9
通讯作者：
Jie Chen
中科院分区：
文献类型：
--
作者：
Li;S. Evers;Zhaohui Lu;Yan Shen;Jie Chen
我们在这里报告人类胰腺的二维蛋白质数据库。通过二维电泳，然后基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）分析蛋白质。共鉴定出302种蛋白质，其中约27%是具有广泛催化活性的酶。其中几种在胰腺中特异性表达，如胰淀粉酶、胰石蛋白、胰腺炎相关蛋白、胰脂肪酶、胰弹性蛋白酶等。结构和细胞骨架蛋白也在凝胶上强烈表达。因此，胰腺蛋白质组反映了器官的功能。这项工作为进一步研究胰腺蛋白在健康和疾病（如糖尿病和胰腺癌）中的表达铺平了道路。
We report here the two‐dimensional protein database of human pancreas. The proteins were analyzed by two‐dimensional electrophoresis followed by matrix‐assisted laser desorption/ionization‐time of flight‐mass spectrometry (MALDI‐TOF‐MS). Totally, 302 proteins were identified, of which about 27% were enzymes with a broad range of catalytic activities. Several of these are specifically expressed in pancreas, such as pancreatic amylase, pancreatic stone protein, pancreatitis‐associated protein, pancreatic lipase, pancreatic elastase, etc. Structural and cytoskeletal proteins are also strongly represented on the gels. Thus, the pancreatic proteome reflects the organ's function. This work paves the way for further studies on pancreatic protein expression in health and disease, such as diabetes and pancreatic cancer.