Enhanced growth rate under elevated CO2 conditions was observed for transgenic lines of genes identified by intraspecific variation analyses in Arabidopsis thaliana
Enhanced growth rate under elevated CO2 conditions was observed for transgenic lines of genes identified by intraspecific variation analyses in Arabidopsis thaliana
复制标题
通过拟南芥种内变异分析鉴定的转基因品系，观察到在升高的 CO2 条件下生长速度加快
DOI：
10.1007/s11103-022-01265-w
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发表时间：
2022
影响因子：
5.1
通讯作者：
K. Hikosaka
中科院分区：
文献类型：
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作者：
R. Oguchi;Kousuke Hanada;Minami Shimizu;M. Mishio;Hiroshi Ozaki;K. Hikosaka
利用拟南芥生态型的全基因组和生长数据，我们确定了两个与提高CO2条件下的生长速率相关的基因。在CO2浓度升高的条件下改善植物生长可能有助于在未来全球气候变化下提高农业产量。在这项研究中，我们研究了基因牵连在CO2浓度升高的条件下，通过分析来自世界各地的不同纬度和海拔高度的拟南芥生态型的增长率的增长率。我们还进行了全基因组关联研究和转录组研究，以确定与CO2升高条件下的相对生长率（RGR）或RGR的CO2响应相关的单核多态性。然后，我们选择了43个候选基因，并产生了它们的过表达和/或RNA干扰（RNAi）转基因突变体进行筛选。经过筛选，我们发现AT 3G 4000和AT 5G 50900的RNAi株系在高CO2条件下表现出显著更高的生长速率。根据我们的研究结果，我们得出结论，自然变异包括与CO2浓度升高条件下植物生产力提高相关的遗传变异。
Using the whole genome and growth data of Arabidopsis thaliana ecotypes, we identified two genes associated with enhancement of the growth rate in response to elevated CO2 conditions. Improving plant growth under elevated CO2 conditions may contribute to enhanced agricultural yield under future global climate change. In this study, we examined the genes implicated in the enhancement of growth rates under elevated CO2 conditions by analyzing the growth rates of Arabidopsis thaliana ecotypes originating from various latitudes and altitudes throughout the world. We also performed a genome-wide association study and a transcriptome study to identify single nucleic polymorphisms that were correlated with the relative growth rate (RGR) under elevated CO2 conditions or with CO2 response of RGR. We then selected 43 candidate genes and generated their overexpression and/or RNA interference (RNAi) transgenic mutants for screening. After screening, we have found that RNAi lines of AT3G4000 and AT5G50900 showed significantly higher growth rates under the elevated CO2 condition. As per our findings, we conclude that natural variation includes genetic variation associated with the enhancement of plant productivity under elevated CO2 conditions.