Anthocyanins protect against LPS-induced oxidative stress-mediated neuroinflammation and neurodegeneration in the adult mouse cortex
Anthocyanins protect against LPS-induced oxidative stress-mediated neuroinflammation and neurodegeneration in the adult mouse cortex
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DOI：
10.1016/j.neuint.2016.08.005
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发表时间：
2016-11-01
影响因子：
4.2
通讯作者：
Kim, Myeong Ok
中科院分区：
文献类型：
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作者：
Khan, Muhammad Sohail;Ali, Tahir;Kim, Myeong Ok
几项研究提供了证据表明，活性氧物种(ROS)是各种神经疾病的关键介质。花青素是一种多酚化合物，以其抗氧化和神经保护作用而闻名。在这项研究中，我们研究了花色苷(从黑豆中提取)对脂多糖(LPS)诱导的成年小鼠皮层神经炎症和神经变性的保护作用。腹腔注射脂多糖(250微克/公斤)7天可引起ROS升高和氧化应激，从而导致成年小鼠皮质神经炎症和神经变性。与单独注射脂多糖的小鼠相比，给予24 mg/kg/天的花青素治疗14天(之前7天和与内毒素联合处理7天)可以减轻升高的ROS和氧化应激。免疫印迹结果表明，花色苷降低了氧化应激激酶磷酸化c-Jun氨基末端激酶1(p-JNK)的水平。免疫印迹和形态学结果表明，花青素处理通过抑制IL-1β、TNF-α和转录因子NF-B-k等多种炎症介质，显著减轻了内毒素诱导的ROS介导的神经炎症。花青素处理还减少了脂多糖注射小鼠大脑皮质中激活的星形胶质细胞和小胶质细胞，这分别从GFAP和Iba-1的减少来表明。花青素还可以防止多种凋亡标志物的过度表达，即Bax、胞浆细胞色素C、裂解的caspase-3和PARP-1。免疫组织化学FJB和Nissi染色表明，花色苷可防止脂多糖诱导的小鼠皮层神经变性。我们的结果表明，饮食中的类黄酮，如花青素，具有抗氧化和神经保护活性，可能有利于各种神经疾病。(C)2016爱思唯尔有限公司。保留所有权利。
Several studies provide evidence that reactive oxygen species (ROS) are key mediators of various neurological disorders. Anthocyanins are polyphenolic compounds and are well known for their antioxidant and neuroprotective effects. In this study, we investigated the neuroprotective effects of anthocyanins (extracted from black soybean) against lipopolysaccharide (LPS)-induced ROS-mediated neuroinflammation and neurodegeneration in the adult mouse cortex. Intraperitoneal injection of LPS (250 mu g/kg) for 7 days triggers elevated ROS and oxidative stress, which induces neuroinflammation and neurodegeneration in the adult mouse cortex. Treatment with 24 mg/kg/day of anthocyanins for 14 days in LPS-injected mice (7 days before and 7 days co-treated with LPS) attenuated elevated ROS and oxidative stress compared to mice that received LPS-injection alone. The immunoblotting results showed that anthocyanins reduced the level of the oxidative stress kinase phospho-c-Jun N-terminal Kinase 1 (p-JNK). The immunoblotting and morphological results showed that anthocyanins treatment significantly reduced LPS-induced-ROS-mediated neuroinflammation through inhibition of various inflammatory mediators, such as IL-1 beta, TNF-alpha and the transcription factor NF-B-k. Anthocyanins treatment also reduced activated astrocytes and microglia in the cortex of LPS-injected mice, as indicated by reductions in GFAP and Iba-1, respectively. Anthocyanins also prevent overexpression of various apoptotic markers, i.e., Bax, cytosolic cytochrome C, cleaved caspase-3 and PARP-1. Immunohistochemical fluoro-jade B (FJB) and Nissi staining indicated that anthocyanins prevent LPS-induced neurodegeneration in the mouse cortex. Our results suggest that dietary flavonoids, such as anthocyanins, have antioxidant and neuroprotective activities that could be beneficial to various neurological disorders. (C) 2016 Elsevier Ltd. All rights reserved.