Suppression of Bta11975, an α-glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci
Suppression of Bta11975, an α-glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci
复制标题
通过 RNA 干扰抑制 Bta11975（一种 α-葡萄糖苷酶）可减少烟粉虱传播番茄褪绿病病毒
DOI：
10.1002/ps.6572
复制
发表时间：
2021-08-09
影响因子：
4.1
通讯作者：
Liu, Yong
中科院分区：
文献类型：
--
作者：
Lu, DingYiHui;Yue, Hao;Liu, Yong
背景技术背景：番茄褪绿病病毒（ToCV）在我国主要由烟粉虱（Bemisia tabaci）传播，在世界范围内均有分布，严重影响番茄等蔬菜的产量。目前，ToCV的控制主要集中在使用杀虫剂控制粉虱种群。转录组测序显示B的高表达。烟粉虱Bta 11975基因，地中海烟粉虱（MED）物种在ToCV获取过程中的α-葡萄糖苷酶（AGLU）。目的探讨Bta 11975基因在B获得和传播ToCV中的作用。结果：Bta 11975基因的相对表达量与B中ToCV含量呈显著正相关。烟粉虱。AGLU在初级唾液腺和肠道中高度表达。Bta 11975基因沉默后，B.烟粉虱减少，B.烟粉虱死亡率增加。此外，ToCV被B收购。B的浓度为25或50时，烟粉虱在48和72 h AAP时的ToCV传播显著降低。结论：这些结果表明Bta 11975基因在B.利用RNAi技术对烟粉虱MED基因进行改造，可以减少B对ToCV的获取和传播。烟粉虱
BACKGROUND: Tomato chlorosis virus (ToCV) is mainly vectored by Bemisia tabaci in China, which has a worldwide distribution, and greatly reduces the yields of tomato and other vegetables. At present, control of ToCV has been focused mainly by the use of insecticides to control whitefly populations. Transcriptome sequencing showed high expression of the B. tabaci Bta11975 gene, an alpha-glucosidase (AGLU) during ToCV acquisition by whitefly Mediterranean (MED) species. To investigate the role of Bta11975 gene in ToCV acquisition and transmission by B. tabaci MED, we used RNA interference (RNAi) to reduce the expression of the Bta11975 gene.RESULTS: The relative expression of the Bta11975 gene was correlated with the ToCV content in B. tabaci. The AGLU is highly expressed in primary salivary gland and gut. After the Bta11975 gene was silenced, the gene expression of B. tabaci was reduced and B. tabaci mortality was increased. Besides, ToCV acquisition by B. tabaci at 48 and 72 h AAP was reduced, and ToCV transmission was significantly reduced by 25 or 50 of B. tabaci.CONCLUSIONS: These results indicate that suppression of expression of the Bta11975 gene in B. tabaci MED by RNAi can reduce acquisition and transmission of ToCV by B. tabaci MED.