Expression of AT1-R in marmoset whole adrenal glands and adrenocortical cells in culture.
Expression of AT1-R in marmoset whole adrenal glands and adrenocortical cells in culture.
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AT1-R 在狨猴整个肾上腺和培养的肾上腺皮质细胞中的表达。
DOI：
10.1081/erc-200044025
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发表时间：
2004
影响因子：
2.1
通讯作者：
Pattison,JChristina
中科院分区：
文献类型：
--
作者：
Bird,IanM;Pattison,JChristina
狨猴肾上腺很有趣，因为它在发育上与人类和其他灵长类动物相似，但在成年期 17,20-裂解酶活性非常低。一种可能的解释是 AT1-R 对 P450c17 表达的调节发生了改变。我们研究了 AT1-R 在狨猴肾上腺和培养的肾上腺皮质细胞中的表达和区域分布，因为已知 AT1-R 是 P450c17 表达和活性的调节因子。 AT1-R 在 ZG 中强烈表达，并在皮质的其余部分表达减弱。假定的 ZI 中存在负面表达。 ICC 检测到分散的肾上腺皮质细胞保留了 AT1-R 蛋白。细胞培养数天并在无血清培养基中处理 48 小时，通过 Western 测量，维持 AT1-R 表达。然而，在该 48 小时时间点，用 Forsk、AII、TPA 或 A + F 或 T + F 组合治疗似乎不会影响 AT1-R 表达。我们得出结论，狨猴肾上腺表达 AT1-R 与人类和其他高等哺乳动物相似，肾上腺皮质细胞在培养物中保留 AT1-R 表达，并且与其他肾上腺细胞培养模型一致，AT1-R 表达不会因长期激动剂的反应而丢失。
The marmoset adrenal is interesting as it is developmentally similar to humans and other primates, but in adulthood 17,20‐lyase activity is very low. One possible explanation is an altered regulation of P450c17 expression by AT1‐R. We investigated the expression and zonal distribution of the AT1‐R in marmoset adrenal glands and adrenocortical cells in culture since it is known that AT1‐R is a regulator of P450c17 expression and activity. AT1‐R was expressed strongly in the ZG and to diminishing degrees through the remainder of the cortex. There was negative expression in a putative ZI. Dispersed adrenocortical cells retained AT1‐R protein as detected by ICC. Cells cultured for several days and treated in serum‐free media for 48 h maintained AT1‐R expression, as measured by western. However, at that 48 h time point, treatment with Forsk, AII, TPA, or the combination of A + F or T + F did not appear to effect AT1‐R expression. We conclude that marmoset adrenals express AT1‐R similarly to humans and other higher mammals, adrenocortical cells retain AT1‐R expression in culture, and consistent with other adrenal cell culture models, AT1‐R expression is not lost in response to agonists long term.