DNA Methylation is Correlated with Pluripotency of Stem Cells.
DNA Methylation is Correlated with Pluripotency of Stem Cells.
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DOI：
10.2174/1574888x11666161226145432
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发表时间：
2017-07
影响因子：
2.7
通讯作者：
Ruofeng Wang;Tianqing Li
中科院分区：
文献类型：
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作者：
Ruofeng Wang;Tianqing Li
背景干细胞的多能性是一个重要的科学问题，引起了社会各界的极大兴趣，尤其是在再生医学领域。哺乳动物的多能干细胞(PSCs)根据其细胞、分子、表观遗传和功能状态被定义为初始状态和启动状态。目的了解干细胞DNA甲基化与多能性的关系。方法在已发表文献的基础上，对胚胎干细胞的DNA甲基化及其功能进行了讨论。我们还总结了DNA甲基化与幼稚状态维持的关系，并展望了未来灵长类幼稚PSCs的DNA甲基化研究重点。结果与结论DNA甲基化与PSCs的细胞重编程、功能重塑及细胞分化密切相关。PSCs的多能性和幼稚特性与细胞DNA甲基化密切相关。然而，由于缺乏广泛接受的培养条件，涉及幼稚地甲基化修饰的机制仍然是灵长类幼稚PSCs的重要科学问题之一。
BACKGROUND Pluripotency of stem cells is an important scientific issue and is attracting great interest for the broader community, especially for regenerative medicine field. Pluripotent stem cells (PSCs) in mammalians are defined as naive- and primed-states according to their cellular, molecular, epigenetic and functional states. OBJECTIVE Understand the correlation between DNA methylation and pluripotency of stem cells. METHOD Based on published papers, we discussed the DNA methylation and corresponding functions for embryonic stem cells. We also summarized the correlation between DNA methylation and naive state maintenance, and outlook future emphasis of DNA methylation for primate naive PSCs. RESULTS AND CONCLUSION DNA methylation is closely associated with cell reprogramming, functional remodeling and cell differentiation of PSCs. The pluripotency and naive characteristics of PSCs are closely associated with cell DNA methylation. However, the mechanisms, which are involved in methylation modifications of naive ground, are still one of the important scientific issues for primate naive PSCs because of lack of widely accepted culture condition.