Sensitivity of sixteen APTT reagents for the presence of lupus anticoagulants.
Sensitivity of sixteen APTT reagents for the presence of lupus anticoagulants.
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十六种 APTT 试剂对狼疮抗凝剂存在的敏感性。
DOI：
10.1159/000217148
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
Elisabeth Verdy
中科院分区：
文献类型：
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作者：
A. Denis;Antoine Flahault;Elisabeth Verdy
活化部分凝血活酶时间（APTT）是最常用的检测用于筛选狼疮抗凝剂（LA）。多年来，制造商一直在提出对LA更敏感的APTT试剂。本研究评价了16种APTT试剂在40例LA和46例正常人血浆中的敏感性和反应性。使用正常血浆和LA血浆的1：1混合物确定灵敏度，优化了最灵敏的试剂。使用相同的正常血浆池进行混合物测定，并使用每日APTT值确定比值。结果表明，新的市售APTT试剂对LA更敏感和响应。不同APTT试剂的灵敏度差异可能部分是由于LA的异质性，因此应使用多种APTT试剂进行LA检测。APTT试剂的选择是LA评估的重要初步步骤。
Activated partial thromboplastin time (APTT) is the most common assay used to screen for lupus anticoagulant (LA). For some years, manufacturers have been proposing APTT reagents that were more sensitive to LA. In this study, we evaluated the sensitivity and responsiveness of 16 APTT reagents in 40 LA and 46 normal plasmas. Sensitivity was established using a 1:1 mixture of normal and LA plasma that optimized the most sensitive reagent. The same pool of normal plasma was used for the assay of mixtures and daily APTT values for the determination of ratios. The results show that new commercially available APTT reagents are more sensitive and responsive to LA. The different sensitivities of APTT reagents might be partly due to the heterogeneity of LAs and several APTT reagents should be used for LA detection. The choice of APTT reagents constitutes an important preliminary step for LA evaluation.