Plasma lipidomics reveals potential prognostic signatures within a cohort of cystic fibrosis patients
Plasma lipidomics reveals potential prognostic signatures within a cohort of cystic fibrosis patients
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DOI：
10.1194/jlr.p013722
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发表时间：
2011-05-01
影响因子：
6.5
通讯作者：
Edelman, Aleksander
中科院分区：
文献类型：
--
作者：
Ollero, Mario;Astarita, Giuseppe;Edelman, Aleksander
囊性纤维化与脂代谢异常有关。我们最近发现，几种磷脂酰胆碱(PC)和溶血磷脂酰胆碱(LPC)的血浆水平变化与CF患者的疾病严重程度有关。我们的目的是寻找携带相同突变(F508del)和不同表型的CF患者的血脂特征，并研究它们与S 1号用力呼气量(FEV 1)和铜绿假单胞菌慢性感染的相关性，在测试时(t=0)和3年后(t=3)进行评估。对44个F508del纯合子的样品进行了基于LC-ESI-MS的脂组学方法。12种游离脂肪酸与FEV1呈正相关，t=0(n=29)。其中4例(C20：3n-9、C20：5n-3、C22：5n-3、C22：6n-3)与PC(32：2)、PC(36：4)(n=31)3年后与FEV1呈正相关。油酰乙醇胺(OEA)与FEV1分级呈负相关(n=17)。T=0时，慢性感染者的PC(32：2)、PC(38：5)和C18：3n-3较低，而胆固醇、胆固醇酯和三酰甘油(TAG)较高。T=3的慢性感染者LPC水平显著降低(18：0)。这些结果表明，在我们所知的第一项旨在确定CF.-Ollero，M.，G.Astarita，I.C.Guerrera，I.Sermet-Gaudelus，S.Trudel，D.Piomelli和A.Edelman的脂质生物标记物的纵向研究中，一些脂质标记具有潜在的预后价值。血浆脂组学在囊性纤维化患者队列中显示了潜在的预后标志。J.Lipid Res.2011年。52：1011-1022。
Cystic fibrosis (CF) is associated with abnormal lipid metabolism. We have recently shown variations in plasma levels of several phosphatidylcholine (PC) and lyso-phopshatidylcholine (LPC) species related to disease severity in CF patients. Here our goal was to search for blood plasma lipid signatures characteristic of CF patients bearing the same mutation (F508del) and different phenotypes, and to study their correlation with forced expiratory volume in 1 s (FEV1) and Pseudomonas aeruginosa chronic infection, evaluated at the time of testing (t = 0) and three years later (t = 3). Samples from 44 F508del homozygotes were subjected to a lipidomic approach based on LC-ESI-MS. Twelve free fatty acids were positively correlated with FEV1 at t = 0 (n = 29). Four of them (C20:3n-9, C20:5n-3, C22:5n-3, and C22:6n-3) were also positively correlated with FEV1 three years later, along with PC(32: 2) and PC(36: 4) (n = 31). Oleoylethanolamide (OEA) was negatively correlated with FEV1 progression (n = 17). Chronically infected patients at t = 0 showed lower PC(32: 2), PC(38: 5), and C18:3n-3 and higher cholesterol, cholesterol esters, and triacylglycerols (TAG). Chronically infected patients at t = 3 showed significantly lower levels of LPC(18: 0). These results suggest a potential prognostic value for some lipid signatures in, to our knowledge, the first longitudinal study aimed at identifying lipid biomarkers for CF.-Ollero, M., G. Astarita, I. C. Guerrera, I. Sermet-Gaudelus, S. Trudel, D. Piomelli, and A. Edelman. Plasma lipidomics reveals potential prognostic signatures within a cohort of cystic fibrosis patients. J. Lipid Res. 2011. 52: 1011-1022.