IL-28B rs12979860 C/T allele distribution in patients with liver cirrhosis: Role in the course of chronic viral hepatitis and the development of HCC
IL-28B rs12979860 C/T allele distribution in patients with liver cirrhosis: Role in the course of chronic viral hepatitis and the development of HCC
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DOI：
10.1016/j.jhep.2010.07.019
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发表时间：
2011-04-01
影响因子：
25.7
通讯作者：
Toniutto, Pierluigi
中科院分区：
文献类型：
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作者：
Fabris, Carlo;Falleti, Edmondo;Toniutto, Pierluigi
背景与目的：白细胞介素28B （IL-28B）基因上游3kb的单核苷酸多态性（rs12979860 C/T）被证明与丙型肝炎清除有关。我们验证了这种关联是否也转化为疾病轨迹结束时不同的基因型分布。方法：采用RFLP-PCR技术对412例丙型肝炎（n = 199）、乙型肝炎（n = 75）、酒精（n = 110）及其他原因肝硬化（n = 28）患者进行基因分型，其中256例行肝移植。肝细胞癌（HCC）在85例OLT患者的原生肝脏中被证实，52例为病毒性肝硬化，33例为非病毒性肝硬化。292例轻度慢性肝炎患者（235例HCV阳性，57例HBV阳性）和344名健康受试者作为对照。结果：病毒性肝硬化患者（C/C = 99, C/T = 137, T/T = 38）与非病毒性肝硬化患者（C/C = 72, C/T = 58, T/T = 8） IL-28B rs12979860基因型频率差异有统计学意义（p = 0.0005）。与轻度丙型肝炎或hbv相关肝硬化相比，HCV相关肝硬化患者携带T/T基因型的频率更高。IL-28B rs12979860基因型频率在肝硬化合并HCC的OLT患者中为C/C = 23, C/T = 50, T/T = 12，在肝硬化不合并HCC的患者中为C/C = 79, C/T = 78, T/T = 14 (p
Background & Aims: A single nucleotide polymorphism (rs12979860 C/T) 3 kb upstream of the interleukin 28B (IL-28B) gene was shown to be associated with hepatitis C clearance. We verified whether this association also translates into a different genotype distribution at the end of the disease trajectory.Methods: A RFLP-PCR technique was used to genotype 412 patients with cirrhosis due to hepatitis C (n = 199), hepatitis B (n = 75), alcohol (n = 110), and other causes (n = 28), of whom 256 underwent liver transplantation (OLT). Hepatocellular carcinoma (HCC) was demonstrated in the native liver of 85 OLT patients, 52 with viral cirrhosis, and 33 with non-viral cirrhosis respectively. A group of 292 patients (235 HCV and 57 HBV positive) with mild chronic hepatitis and 344 healthy subjects served as controls.Results: A significant difference (p = 0.0005) was observed in IL-28B rs12979860 genotype frequencies between patients with viral cirrhosis (C/C = 99, C/T = 137, T/T = 38) and those with non-viral cirrhosis (C/C = 72, C/T = 58, T/T = 8). Patients with HCV related cirrhosis carried more frequently the T/T genotype in comparison to mild hepatitis C or HBV-related cirrhosis. IL-28B rs12979860 genotype frequencies were C/C = 23, C/T = 50, T/T = 12 among OLT patients with cirrhosis complicated by HCC, and C/C = 79, C/T = 78, T/T = 14 among patients with cirrhosis not complicated by HCC (p