A rhodamine-hydroxamic acid-based fluorescent probe for hypochlorous acid and its applications to biological imagings.
A rhodamine-hydroxamic acid-based fluorescent probe for hypochlorous acid and its applications to biological imagings.
复制标题
DOI：
10.1021/ol802822t
复制
发表时间：
2009-01
期刊：
影响因子：
5.2
通讯作者：
Young-Keun Yang;Hyungseoph J Cho;Jihyun Lee;I. Shin;J. Tae
中科院分区：
文献类型：
--
作者：
Young-Keun Yang;Hyungseoph J Cho;Jihyun Lee;I. Shin;J. Tae
建立了一种新的罗丹明-异羟肟酸荧光化学传感器，用于水溶液中HOCl的快速检测。该系统，它利用了不可逆的HOCl促进的氧化反应，在室温下与HOCl的量成线性比例的瞬时响应。该系统对HOCl的选择性高于其他活性氧物质（ROS），并且在水溶液中高度敏感。使用活细胞和生物体（A549细胞和斑马鱼）检测HOCl的生物成像研究被成功地证明。
A new rhodamine-hydroxamic acid-based fluorescent chemosensor for the rapid detection of HOCl in aqueous media was developed. The system, which utilizes an irreversible HOCl-promoted oxidation reaction, responds instantaneously at room temperature with linear proportionality to the amount of HOCl. This system is highly selective for HOCl over other reactive oxygen species (ROS) and highly sensitive in aqueous solutions. Biological imaging studies using living cells and organisms (A549 cells and zebrafish) to detect HOCl are successfully demonstrated.