Biosensing platform for the detection of uric acid based on graphene quantum dots and G-quadruplex/hemin DNAzyme
Biosensing platform for the detection of uric acid based on graphene quantum dots and G-quadruplex/hemin DNAzyme
复制标题
DOI：
10.1016/j.aca.2017.01.067
复制
发表时间：
2017-05-01
影响因子：
6.2
通讯作者：
Su, Xingguang
中科院分区：
文献类型：
--
作者：
Cai, Nan;Tan, Lu;Su, Xingguang
本文利用g -四联体/血红素DNAzyme的过氧化物酶模拟活性和咖啡酸的引入，设计了无标记的尿酸荧光检测生物传感平台。尿酸可被尿酸酶分解，生成过氧化氢和尿囊素。因此，我们成功地实现了通过监测过氧化氢浓度间接检测尿酸。g -四联体/血红蛋白DNAzyme可以作为过氧化物酶，在室温下将过氧化氢分解成羟基自由基。由于羟基自由基的强氧化作用，咖啡酸被转化为相应的醌，从而导致GQDs的荧光猝灭。在优化的实验条件下，猝灭荧光强度与尿酸浓度呈线性关系，在2 ~ 300 mM范围内，检出限为500 nM。进一步证明了该方法的适用性，在人血清和尿液样本中取得了满意的结果。(C) 2017年Elsevier b.v.出版。
In this paper, a label-free biosensing platform for fluorescence detection of uric acid was designed on the peroxidase-mimicking activities of G-quadruplex/hemin DNAzyme and the introduction of caffeic acid. Uric acid could be decomposed by uricase and then produced hydrogen peroxide and allantoin. We thus successfully achieved the indirect detection of uric acid by monitoring the concentration of hydrogen peroxide. The G-quadruplex/hemin DNAzyme could act as peroxidase and decompose the hydrogen peroxide into hydroxyl radicals at room temperature. Due to the strong oxidizing of hydroxyl radicals, caffeic acid was converted to corresponding quinone, thus leading to fluorescence quenching of GQDs. Under the optimized experimental conditions, the quenched fluorescence intensity was linearly relative to the concentration of uric acid, ranging from 2 mM to 300 mM with a detection limit of 500 nM. The applicability of proposed method was further proved with satisfactory results in human serum and urine samples. (C) 2017 Published by Elsevier B. V.