The unholy trinity: taxonomy, species delimitation and DNA barcoding
The unholy trinity: taxonomy, species delimitation and DNA barcoding
复制标题
DOI：
10.1098/rstb.2005.1722
复制
发表时间：
2005-10-29
影响因子：
6.3
通讯作者：
Siddall, M
中科院分区：
文献类型：
--
作者：
DeSalle, R;Egan, MG;Siddall, M
最近，一项为地球上所有已命名物种生成DNA序列的计划的发展令人兴奋，在我们看来，这一“DNA条形码”计划应该如何进行，产生了两个主要的争论领域。在使用DNA作为分类和物种划分的工具之前，澄清和解决这两个问题是至关重要的。第一个问题涉及如何在这一倡议的背景下使用DNA数据；这就是DNA条形码读取器问题（或条形码问题）。目前，在该计划下发表的许多研究都使用了树构建方法和更精确的距离方法来构建树，这些树用于将某些DNA序列置于分类环境中。第二个问题涉及到分类学界对“DNA条形码”倡议的反应。这个问题非常重要，因为传统的分类学方法和DNA方法需要调和，以便“DNA条形码”倡议能够得到任何形式的社区接受。事实上，我们觉得DNA条形码是一个用词不当。我们更喜欢伦敦会议的标题——条形码生活。在本文中，我们讨论了围绕DNA条形码倡议产生的这两个问题，并试图提出一个改进条形码的系统发育系统框架，以及一个将经典分类学与“DNA条形码”目标交织在一起的分类学框架。
Recent excitement over the development of an initiative to generate DNA sequences for all named species on the planet has in our opinion generated two major areas of contention as to how this 'DNA barcoding' initiative should proceed. It is critical that these two issues are clarified and resolved, before the use of DNA as a tool for taxonomy and species delimitation can be universalized. The first issue concerns how DNA data are to be used in the context of this initiative; this is the DNA barcode reader problem (or barcoder problem). Currently, many of the published studies under this initiative have used tree building methods and more precisely distance approaches to the construction of the trees that are used to place certain DNA sequences into a taxonomic context. The second problem involves the reaction of the taxonomic community to the directives of the 'DNA barcoding' initiative. This issue is extremely important in that the classical taxonomic approach and the DNA approach will need to be reconciled in order for the 'DNA barcoding' initiative to proceed with any kind of community acceptance. In fact, we feel that DNA barcoding is a misnomer. Our preference is for the title of the London meetings-Barcoding Life. In this paper we discuss these two concerns generated around the DNA barcoding initiative and attempt to present a phylogenetic systematic framework for an improved barcoder as well as a taxonomic framework for interweaving classical taxonomy with the goals of 'DNA barcoding'.