DETERMINATION OF BORON IN TISSUES AND CELLS USING DIRECT-CURRENT PLASMA ATOMIC EMISSION-SPECTROSCOPY
DETERMINATION OF BORON IN TISSUES AND CELLS USING DIRECT-CURRENT PLASMA ATOMIC EMISSION-SPECTROSCOPY
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DOI：
10.1021/ac00009a010
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发表时间：
1991-05-01
影响因子：
7.4
通讯作者：
ANISUZZAMAN, AKM
中科院分区：
文献类型：
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作者：
BARTH, RF;ADAMS, DM;ANISUZZAMAN, AKM
我们开发了一种安全、简单、高效的直流等离子体原子发射光谱法测定硼的方法。 使用浓硫酸和70%过氧化氢溶解组织来消化样品，无需高温高压。 硼簇化合物的测量灵敏度、精密度和准确度与硼酸标准品相似。 用[(C2H5)3NH]2B12H12、Cs2B12H11SH.H2O和C15H32B10O6获得的结果表明该分析方法适用于多种不同化学结构的化合物。 单独使用已知标准以及在各种组织基质（包括肿瘤、血液、肝脏、皮肤和细胞悬浮液）中均获得了 0.1 ppm 的灵敏度。 通过直流等离子体原子发射光谱 (DCP-AES) 可以用少至 50 mg 的组织或少至 5 x 10(7) 个细胞实现总硼的测量。 该程序适用于 ppm 范围内的硼分析，具有高精度和准确度。
We have developed a safe, simple, and efficient method for boron determination by means of direct-current plasma atomic emission spectroscopy. Tissues were solubilized by using concentrated sulfuric acid and 70% hydrogen peroxide to digest the samples without the need of high temperatures and pressures. Boron cluster compounds could be measured with sensitivity, precision, and accuracy similar to those of boric acid standards. Results obtained with [(C2H5)3NH]2B12H12, Cs2B12H11SH.H2O, and C15H32B10O6 show that this analytical method is applicable to a variety of compounds with different chemical structures. A sensitivity of 0.1 ppm has been obtained with known standards alone and in a variety of tissue matrices including tumor, blood, liver, skin, and cell suspensions. The measurement of total boron by direct-current plasma atomic emission spectroscopy (DCP-AES) has been achieved with as little as 50 mg of tissue or as few as 5 x 10(7) cells. The procedure is applicable to the analysis of boron in the ppm range with a high degree of precision and accuracy.