Sex-related effect on gene expression in the mouse meibomian gland
Sex-related effect on gene expression in the mouse meibomian gland
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DOI：
10.1080/02713680500514644
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发表时间：
2006-02-01
影响因子：
2
通讯作者：
Jensen, RV
中科院分区：
文献类型：
--
作者：
Richards, SM;Yamagami, H;Jensen, RV
目的：性别相关的差异已被确定在解剖和生理的睑板腺。我们假设这些差异是由于，至少部分是由于基因表达的变化。本研究的目的是确定性别相关的差异是否确实存在于睑板腺基因表达。我们还试图阐明这种差异（如果有的话）是否可能（a）类似于已知发生在泪腺中的差异和（B）由于性类固醇的作用。研究方法：从年轻成年雄性和雌性BALB/c小鼠（每个实验每个性别n = 7至15只小鼠）获得睑板腺，根据性别合并并处理用于分离RNA。通过使用CodeLink Bioarrays和GEM 1和GEM 2基因芯片评价样品的差异表达mRNA。用GeneSifter分析生物阵列数据。Net软件，并与GEO和GeneSifter数据库中的微阵列数据进行比较。结果：性别对小鼠睑板腺中164个基因的表达有显著影响。这些基因参与广泛的生物过程、分子功能和细胞组分，包括代谢、催化、细胞生长和维持、膜结构、核酸结合、转录和信号转导等活动。此外，睑板腺基因表达的性别相关变异的性质与泪腺中的差异很大，似乎部分是由雄激素介导的，而不是雌激素或孕激素。结论：这些发现支持了我们的假设，性别相关的差异存在于睑板腺的基因表达。
Purpose : Sex-related differences have been identified in the anatomy and physiology of the meibomian gland. We hypothesize that these differences are due, at least in part, to variations in gene expression. This study's objective was to determine whether sex-related differences do exist in meibomian gland gene expression. We also sought to elucidate whether such differences, if any, might be (a) analogous to those known to occur in the lacrimal gland and (b) due to the effect of sex steroids. Methods : Meibomian glands were obtained from young adult male and female BALB/c mice (n = 7 to 15 mice per sex per experiment), pooled according to sex and processed for the isolation of RNA. Samples were evaluated for differentially expressed mRNAs by using CodeLink Bioarrays and GEM 1 and 2 gene chips. Bioarray data were analyzed with GeneSifter. Net software and also compared with microarray data in GEO and GeneSifter databases. Results : Our results demonstrate that sex has a significant influence on the expression of 164 genes in the mouse meibomian gland. These genes are involved in a broad spectrum of biological processes, molecular functions, and cellular components, including such activities as metabolism, catalysis, cell growth and maintenance, membrane architecture, nucleic acid binding, transcription, and signal transduction. In addition, the nature of the sex-related variations in meibomian gland gene expression is quite different from those in the lacrimal gland and appear to be mediated in part by the action of androgens, but not estrogens or progestins. Conclusions : These findings support our hypothesis that sex-related differences exist in gene expression of the meibomian gland.