Dose-Response Relationships of Polycyclic Aromatic Hydrocarbons Exposure and Oxidative Damage to DNA and Lipid in Coke Oven Workers
Dose-Response Relationships of Polycyclic Aromatic Hydrocarbons Exposure and Oxidative Damage to DNA and Lipid in Coke Oven Workers
复制标题
焦炉工人多环芳烃暴露与DNA和脂质氧化损伤的剂量-反应关系
DOI：
10.1021/es401639x
复制
发表时间：
2013-07-02
影响因子：
11.4
通讯作者：
Guo, Huan
中科院分区：
文献类型：
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作者：
Kuang, Dan;Zhang, Wangzhen;Guo, Huan
在本研究中，我们招募了1333名男性焦炉工人，监测了环境中PAHs的水平，并测量了体内PAR暴露的生物标志物，包括12种尿PAR代谢物和血浆苯并[a]芘-r-7、t-8、t-9、c-10-四羟基四酚-白蛋白（BPDE-Alb）加合物。以及两种氧化生物标志物尿8-羟基脱氧鸟苷（8-OHdG）和8-异前列腺素- f2 α (8-iso-PGF2 α)。我们发现尿PAR代谢物和血浆BPDE-Alb加合物的总浓度在吸烟者和非吸烟者中都与8-OHdG和8-iso-PGF2 α的升高显著相关（均p < 0.05）。除4-羟基菲和8-OHdG （P-trend = 0.108）外，大多数PAR代谢物也存在这种暴露反应效应（P-trend均< 0.01）。此外，经Bonferroni校正后发现，只有尿1-羟基芘与8-OHdG和8-iso-PGF2 α呈正相关（p < 0.005）。我们的研究结果表明，尿Sigma OH-PAHs和血浆BPDE-Alb加合物可导致DNA和脂质氧化损伤的显著剂量相关增加。此外，当多种分析方法不可用时，我们的研究结果表明尿1-羟基芘是评估焦炉工人总多环芳烃暴露和评估氧化损伤的有用生物标志物。
Polycyclic aromatic hydrocarbons (PAHs) are known to induce reactive oxygen species and oxidative stress, but the dose response relationships between exposure to PAHs and oxidative stress levels have not been established In this study, we recruited 1333 male coke oven workers, monitored the levels of environmental PAHs, and measured internal PAR exposure biomarkers including 12 urinary PAR metabolites and plasma benzo [a] pyrene-r-7,t-8,t-9,c-10-tetrahydotetrol-albumin (BPDE-Alb) adducts, as well as the two oxidative biomarkers urinary 8-hydroxydeoxyguanosine (8-OHdG) and 8-iso-prostaglandin-F2 alpha (8-iso-PGF2 alpha). We found that the total concentration of urinary PAR metabolites and plasma BPDE-Alb adducts were both significantly associated with increased 8-OHdG and 8-iso-PGF2 alpha in both smokers and nonsmokers (all p < 0.05). This exposure response effect was also observed for most PAR metabolites (all P-trend < 0.01), except for 4-hydroxyphenanthrene and 8-OHdG (P-trend = 0.108). Furthermore, it was shown that only urinary 1-hydroxypyrene has a significant positive association with both 8-OHdG and 8-iso-PGF2 alpha after a Bonferroni correction (p < 0.005). Our results indicated that urinary Sigma OH-PAHs and plasma BPDE-Alb adducts can result in significant dose-related increases in oxidative damage to DNA and lipids. Furthermore, when a multianalyte method is unavailable, our findings demonstrate that urinary 1-hydroxypyrene is a useful biomarker for evaluating total PAHs exposure and assessing oxidative damage in coke oven workers.