Molecular characteristics and anti-inflammatory activity of the fucoidan extracted from Ecklonia cava
Molecular characteristics and anti-inflammatory activity of the fucoidan extracted from Ecklonia cava
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DOI：
10.1016/j.carbpol.2012.03.056
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发表时间：
2012-06-20
影响因子：
11.2
通讯作者：
Jeon, You-Jin
中科院分区：
文献类型：
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作者：
Lee, Seung-Hong;Ko, Chang-Ik;Jeon, You-Jin
酶法提取已成功地用于从各种海藻中提取许多生物活性化合物。在这项研究中，我们发现酶法提取褐藻多糖可能比水提取更有利。因此，我们对酶法提取的岩藻胶进行了研究，并采用离子交换色谱法测定了其分子特性和抗炎活性。粗的和分离的岩藻多糖（F-1、F-2和F-3）主要由碳水化合物（47.1-57.1%）、糖醛酸（9.0-15.8%）和硫酸盐（16.5-39.1%）以及不同水平的蛋白质（1.3-8.7%）组成。单糖含量差异显著，主要为焦糖（53.1-77.9%）和半乳糖（10.1-32.8%），鼠李糖（2.3-4.5%）、木糖（4.0-8.2%）和葡萄糖（0.8-2.2%）。这些岩藻糖含有一个或两个亚组分，平均分子量（M-w）从18到359 × 10(3) g/mol不等。这些岩藻胶通过下调iNOS、COX-2和促炎细胞因子如tnf - α、IL-6和IL-1 β的表达，显著抑制脂多糖（LPS）诱导的Raw 264.7巨噬细胞NO的产生。因此，目前的结果表明，褐藻聚糖可能是一种潜在的有用的治疗各种炎症性疾病的方法。(C) 2012 Elsevier Ltd.版权所有。
Enzymatic extraction has been successfully used for extracting numerous biologically active compounds from a wide variety of seaweeds. In this study, we found that enzymatic extraction of the fucoidan from Ecklonia cava may be more advantageous than water extraction. Therefore, we studied the E. cava fucoidans extracted by the enzymatic extraction technique and used ion-exchange chromatography to determine their molecular characteristics and anti-inflammatory activities. The crude and fractionated fucoidans (F-1, F-2, and F-3) consisted mostly of carbohydrates (47.1-57.1%), uronic acids (9.0-15.8%), and sulfates (16.5-39.1%), as well as varying levels of proteins (1.3-8.7%). The monosaccharide levels significantly differed, and the composition included fucose (53.1-77.9%) and galactose (10.1-32.8%), with a small amount of rhamnose (2.3-4.5%), xylose (4.0-8.2%), and glucose (0.8-2.2%). These fucoidans contained one or two subfractions with an average molecular weight (M-w) ranging from 18 to 359 x 10(3) g/mol. These fucoidans significantly inhibited NO production in lipopolysaccharide (LPS)-induced Raw 264.7 macrophage cells by down-regulating the expression of iNOS, COX-2, and pro-inflammatory cytokines such as TNF-alpha, IL-6, and IL-1 beta. Thus, the present results suggest that E. cava fucoidan may be a potentially useful therapeutic approach for various inflammatory diseases. (C) 2012 Elsevier Ltd. All rights reserved.