Failure of isolated rat tibial periosteal cells to 5α reduce testosterone to 5α‐dihydrotestosterone
Failure of isolated rat tibial periosteal cells to 5α reduce testosterone to 5α‐dihydrotestosterone
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离体大鼠胫骨骨膜细胞未能将 5α 睾酮还原为 5α-二氢睾酮
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
T. Spelsberg
中科院分区：
文献类型：
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作者：
R. Turner;B. Bleiberg;D. Colvard;P. Keeting;G. Evans;T. Spelsberg
胶原酶处理后，从成年雄性大鼠胫骨分离骨膜细胞。从分离的骨膜细胞中提取的总细胞质RNA的北方印迹分析显示，编码成骨细胞标志物蛋白骨钙素（BGP）和1型前胶原的前-原-α2（I）链的基因表达呈阳性。将分离的骨膜细胞与1 nM [3 H]睾酮（[3 H]T）孵育长达240分钟，并通过高效液相色谱法分离反应产物。[3H]5在骨膜细胞培养物的提取物中未检测到α-二氢睾酮（[3 H]DHT）。相反，[3 H]DHT是由来自精囊的细胞以时间依赖性方式产生的。这些结果表明，大鼠胫骨骨膜中的成骨细胞不表达睾酮5α-还原酶活性，并且该组织中雄激素的合成代谢作用不由局部产生的DHT介导。
Periosteal cells were isolated from tibiae of adult male rats after collagenase treatment. Northern blot analysis of total cytoplasmic RNA extracted from the isolated periosteal cells was positive for expression of genes encoding the osteoblast marker proteins osteocalcin (BGP) and pre‐pro‐α2(I) chain of type 1 procollagen. The isolated periosteal cells were incubated with 1 nM [3H]testosterone ([3H]T) for up to 240 minutes and the reaction products separated by high‐performance liquid chromatography. [3H]5α‐dihydrotestosterone ([3H]DHT) was not detected in extracts of periosteal cell incubations. In contrast, [3H]DHT was produced in a time‐dependent manner by cells from seminal vesicles. These results suggest that testosterone 5α‐reductase activity is not expressed by osteoblasts in rat tibial periosteum and that the anabolic effects of androgens in this tissue are not mediated by locally produced DHT.