Otefin, a nuclear membrane protein, determines the fate of germline stem cells in Drosophila via interaction with smad complexes
Otefin, a nuclear membrane protein, determines the fate of germline stem cells in Drosophila via interaction with smad complexes
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Otefin 是一种核膜蛋白，通过与 Smad 复合物相互作用来决定果蝇生殖干细胞的命运。
DOI：
10.1016/j.devcel.2008.02.018
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发表时间：
2008-04-01
影响因子：
11.8
通讯作者：
Chen, Dahua
中科院分区：
文献类型：
--
作者：
Jiang, Xiaoyong;Xia, Laixin;Chen, Dahua
核膜蛋白在染色质组织、基因调控和信号转导中发挥重要作用，然而，这些蛋白的生理作用仍然是难以捉摸的。我们发现，otefin（ote），它编码核纤层蛋白，是生殖系干细胞（GSC）的维护所必需的。我们表明，Ote，作为一个内在的因素，是必要的和足够的调节GSC的命运。此外，我们证明了OTE是Dpp/BMP信号通路沉默谷仓转录所必需的。通过结构-功能分析，我们证明了Ote的核膜定位是其在GSC维持中的作用所必需的。最后，我们表明，Ote物理相互作用与美狄亚/Smad 4在谷仓沉默元件，以调节GSC的命运。因此，我们证明了特定的核膜成分介导信号依赖的转录效应来控制干细胞的行为。
Nuclear envelope proteins play important roles in chromatin organization, gene regulation, and signal transduction; however, the physiological role of these proteins remains elusive. We found that otefin (ote), which encodes a nuclear lamin, is essential for germline stem cell (GSC) maintenance. We show that Ote, as an intrinsic factor, is both necessary and sufficient to regulate GSC fate. Furthermore, we demonstrate that ote is required for the Dpp/BMP signaling pathway to silence barn transcription. By structure-function analysis, we demonstrate that the nuclear membrane localization of Ote is essential for its role in GSC maintenance. Finally, we show that Ote physically interacts with Medea/Smad4 at the barn silencer element to regulate GSC fate. Thus, we demonstrate that specific nuclear membrane components mediate signal -dependent transcriptional effects to control stem cell behavior.