Salivary N-glycosyalation as a biomarker of Oral Cancer: A pilot study.
Salivary N-glycosyalation as a biomarker of Oral Cancer: A pilot study.
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唾液 N-糖基化作为口腔癌的生物标志物：一项试点研究。
作者：
N. Sinevici;Stefan Mittermayer;G. Davey;J. Bones;J. O’Sullivan
可靠的口腔癌生物标志物仍然很少，并且用于检测癌前病变的常规测试在临床环境中不是常规的。这项研究解决了目前未满足的需要，更敏感和定量的工具，用于口腔癌的管理。全唾液用于鉴定和鉴定唾液中存在的聚糖的性质，并确定其作为口腔癌生物标志物的潜力。对从全唾液获得的蛋白进行PNGase F酶消化。使用弱阴离子交换色谱法、外切糖苷酶消化结合超高效液相色谱法（UHPLC）和/或质谱法（MS）分析所得N-聚糖。为了确定N-聚糖的变化，使用HILIC-UPLC分析了23名有或没有口腔癌病变的个体，并进行了基于峰面积的相对定量。一个丰富和复杂的唾液N-糖谱被确定。唾液中存在的主要结构是由高甘露糖组成的中性低聚糖，混合和复杂的结构，其次是较小部分的单和二唾液酸化结构。为了确定差异N-糖基化模式是否区分口腔癌和对照组，使用Mann-Whitney检验和主成分分析（PCA）。11个峰显示具有统计学显著性（p ≤ 0.05），而PCA分析显示两组基于其聚糖谱分离。N-糖基化变化在口腔致癌过程中是活跃的，并且可以作为早期检测的生物标志物以降低发病率和死亡率。确定哪些N-聚糖在致癌过程中起最大作用，可能会导致它们在OC的检测，预后和治疗中的应用。
Reliable biomarkers for oral cancer remain scarce and routine tests for the detection of pre-cancerous lesions are not routine in the clinical setting. This study addresses a current unmet need for more sensitive and quantitative tools for the management of oral cancer. Whole saliva was used to identify and characterise the nature of glycans present in saliva and determine their potential as oral cancer biomarkers. Proteins obtained from whole saliva were subjected to PNGase F enzymatic digestion. The resulting N-glycans were analysed with weak anion exchange chromatography, exoglycosidase digestions coupled to ultra-high performance liquid chromatography (UHPLC) and/or mass spectrometry (MS). To determine N-glycan changes, 23 individuals with or without cancerous oral lesions were analysed using HILIC-UPLC and peak based area relative quantitation was performed. An abundant and complex salivary N-glycomic profile was identified. The main structures present in saliva were neutral oligosaccharides consisting of high mannose, hybrid and complex structures, followed by smaller fractions of mono and di-sialylated structures. To determine if differential N-glycosylation patterns distinguish between oral cancer and control groups, Mann-Whitney testing and Principle Component Analysis (PCA) were used. 11 peaks were shown to be statistically significant (p ≤ 0.05) while PCA analysis showed segregation of the two groups based on their glycan profile. N-glycosylation changes are active in the oral carcinogenic process, and may serve as biomarkers for early detection to reduce morbidity and mortality. Identifying which N-glycans contribute most in the carcinogenic process, may lead to their use in the detection, prognosis and treatment of OC.
唾液：一种用于早期检测疾病的新兴生物流体。
影响因子：
1.4
作者：
Lee,Yu-Hsiang;Wong,DavidT
通讯作者：
Wong,DavidT