THE DEVELOPMENT OF A POPULATION OF SPINAL-CORD NEURONS AND THEIR AXONAL PROJECTIONS REVEALED BY GABA IMMUNOCYTOCHEMISTRY IN FROG EMBRYOS
THE DEVELOPMENT OF A POPULATION OF SPINAL-CORD NEURONS AND THEIR AXONAL PROJECTIONS REVEALED BY GABA IMMUNOCYTOCHEMISTRY IN FROG EMBRYOS
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DOI：
10.1098/rspb.1987.0069
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发表时间：
1987-11-23
期刊：
影响因子：
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通讯作者：
STORMMATHISEN, J
中科院分区：
文献类型：
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作者：
DALE, N;ROBERTS, A;STORMMATHISEN, J
使用免疫细胞化学方法鉴定固定神经组织中的GABA，跟踪了爪蟾胚胎脊髓中脑脊液接触神经元群体（“Kolmer-Agduhr”细胞）的发育。含有GABA的染色Kolmer-Agduhr细胞在25期首次出现，其数量随着胚胎发育年龄的增加而稳步增加。Kolmer-Agduhr神经元具有上升的同侧轴突，通常终止于生长锥。这些轴突和生长锥可以用GABA抗血清染色，从Kolmer-Agduhr细胞体最早的生长阶段开始。我们测量了最早的轴突相对于脊髓背尾轴的生长角度。轴突的最初生长总是在一个狭窄的角度范围内吻侧。这一观察结果与随机初始生长的假设不一致，随后选择正确的方向，这将预测轴突最初会在很大的角度范围内生长。相反，它表明，甚至从最早的时刻开始，Kolmer-Agduhr细胞的轴突生长就以一种特定的刻板方式向外定向。
The development of a population of cerebrospinal-fluid-contacting neurons in the spinal cord of theXenopusembryo (‘Kolmer–Agduhr’ cells) has been followed by using an immunocytochemical procedure that identifies GABA in fixed nervous tissue. Stained Kolmer–Agduhr cells containing GABA first appeared at stage 25 and their numbers increased steadily with the developmental age of the embryo. The Kolmer–Agduhr neurons had ascending ipsilateral axons that often terminated in growth cones. These axons and growth cones could be stained by the GABA antiserum from the earliest stages of outgrowth from the Kolmer–Agduhr cell body. We measured the angle of the earliest axons’ outgrowth relative to the rostrocaudal axis of the spinal cord. The initial outgrowth of axons was always rostral over a narrow range of angles. This observation is inconsistent with the hypothesis of random initial outgrowth followed by later selection of the correct orientation, which would predict that axons would initially grow out over a wide range of angles. Instead, it suggests that, even from the earliest moments, axon outgrowth from the Kolmer–Agduhr cells is directed rostrally in a specific stereotyped manner.