A role for adaptor protein-3 complex in the organization of the endocytic pathway in Dictyostelium
A role for adaptor protein-3 complex in the organization of the endocytic pathway in Dictyostelium
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DOI：
10.1111/j.1600-0854.2006.00478.x
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发表时间：
2006-11-01
期刊：
影响因子：
4.5
通讯作者：
Cosson, Pierre
中科院分区：
文献类型：
--
作者：
Charette, Steve J.;Mercanti, Valentina;Cosson, Pierre
盘基网柄菌细胞不断内化细胞外物质，这些物质积累在特征明显的内吞液泡中。在这项研究中，我们描述了一个新的内吞隔室确定的存在下，一个特定的标志物，p25蛋白。这个隔间呈现出让人想起哺乳动物再循环内体的特征：它定位于近中心体区域，但与高尔基体不同。它特别含有表面蛋白质，这些蛋白质被连续内吞，但迅速再循环到细胞表面，因此不存在于成熟的内吞区室中。我们通过研究相应突变体的表型，评估了每个网格蛋白相关接头复合物在建立区室化内吞系统中的重要性。在AP-3网格蛋白相关复合物的亚基μ 3的敲除细胞中，通常限制在p25阳性核内体的膜蛋白被错误定位到晚期内吞隔室。我们的研究结果表明，AP-3起着至关重要的作用，在区室化的内吞途径在网骨藻。
Dictyostelium discoideum cells continuously internalize extracellular material, which accumulates in well-characterized endocytic vacuoles. In this study, we describe a new endocytic compartment identified by the presence of a specific marker, the p25 protein. This compartment presents features reminiscent of mammalian recycling endosomes: it is localized in the pericentrosomal region but distinct from the Golgi apparatus. It specifically contains surface proteins that are continuously endocytosed but rapidly recycled to the cell surface and thus absent from maturing endocytic compartments. We evaluated the importance of each clathrin-associated adaptor complex in establishing a compartmentalized endocytic system by studying the phenotype of the corresponding mutants. In knockout cells for mu 3, a subunit of the AP-3 clathrin-associated complex, membrane proteins normally restricted to p25-positive endosomes were mislocalized to late endocytic compartments. Our results suggest that AP-3 plays an essential role in the compartmentalization of the endocytic pathway in Dictyostelium.