Ultrafast, large-field multiphoton microscopy based on an acousto-optic deflector and a spatial light modulator.
Ultrafast, large-field multiphoton microscopy based on an acousto-optic deflector and a spatial light modulator.
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DOI：
10.1364/ol.37.002532
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发表时间：
2012-07-01
期刊：
影响因子：
3.6
通讯作者：
Gao BZ
中科院分区：
文献类型：
--
作者：
Shao Y;Qin W;Liu H;Qu J;Peng X;Niu H;Gao BZ
介绍了一种基于二维声光偏转器(AOD)扫描器和空间光调制器(SLM)的超快、大视场、高横向和轴向分辨率的多光子激发荧光显微镜。当使用纯位相型单反射光调制器将锁模钛宝石激光器的近红外光整形成包括0级光束的多聚焦阵列时，使用不带任何机械扫描元件的二维AOD扫描器，获得了60倍物镜的136AOD m×136μm视场。使用该系统获得的神经元网络的双光子荧光图像表明，我们的显微镜可以在大范围内以高时间和空间分辨率观察动态生物事件。
We present an ultrafast, large-field multiphoton excitation fluorescence microscope with high lateral and axial resolutions based on a two-dimensional (2-D) acousto-optical deflector (AOD) scanner and spatial light modulator (SLM). When a phase-only SLM is used to shape the near-infrared light from a mode-locked titanium:sapphire laser into a multifocus array including the 0-order beam, a 136 μm × 136 μm field of view is achieved with a 60× objective using a 2-D AOD scanner without any mechanical scan element. The two-photon fluorescence image of a neuronal network that was obtained using this system demonstrates that our microscopy permits observation of dynamic biological events in a large field with high-temporal and -spatial resolution.