Imaging the Recruitment of Cancer-Associated Fibroblasts by Liver-Metastatic Colon Cancer
Imaging the Recruitment of Cancer-Associated Fibroblasts by Liver-Metastatic Colon Cancer
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DOI：
10.1002/jcb.23011
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发表时间：
2011-03-01
影响因子：
4
通讯作者：
Hoffman, Robert M.
中科院分区：
文献类型：
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作者：
Suetsugu, Atsushi;Osawa, Yosuke;Hoffman, Robert M.
肿瘤微环境（TME）对于肿瘤的生长和进展至关重要。然而，TME 的形成很大程度上未知。该报告展示了一种颜色编码的成像模型，可以在其中可视化 TME 的发展。为了对 TME 进行成像，使用表达绿色荧光蛋白 (GFP) 的小鼠作为宿主，该小鼠在所有器官中表达 GFP，但在肝实质细胞中不表达 GFP。将未着色的HCT-116人结肠癌细胞注射到GFP裸鼠的脾脏中，导致实验性肝转移的形成。使用奥林巴斯OV100小动物荧光成像系统观察肝转移引起的TME形成。细胞移植至脾脏后 28 天，HCT-116 细胞在肝脏中形成肿瘤集落。通过荧光成像观察，转移性肿瘤招募了表达 GFP 的宿主细胞。随着时间的推移，结蛋白阳性区域在肝转移灶周围和内部增加，表明肝转移灶招募了癌症相关成纤维细胞（CAF），这些细胞在肿瘤进展中发挥作用。 TME 的颜色编码模型使其形成能够在体内细胞水平上实时可视化。这种 TME 成像模型应该会在 TME 中产生新的视觉目标。 J.细胞。生物化学。 112: 949-953, 2011。(C) 2010 Wiley-Liss, Inc.
The tumor microenvironment (TME) is critical for tumor growth and progression. However, the formation of the TME is largely unknown. This report demonstrates a color-coded imaging model in which the development of the TME can be visualized. In order to image the TME, a green fluorescent protein (GFP)-expressing mouse was used as the host which expresses GFP in all organs but not the parenchymal cells of the liver. Non-colored HCT-116 human colon cancer cells were injected in the spleen of GFP nude mice which led to the formation of experimental liver metastasis. TME formation resulting from the liver metastasis was observed using the Olympus OV100 small animal fluorescence imaging system. HCT-116 cells formed tumor colonies in the liver 28 days after cell transplantation to the spleen. GFP-expressing host cells were recruited by the metastatic tumors as visualized by fluorescence imaging. A desmin positive area increased around and within the liver metastasis over time, suggesting cancer-associated fibroblasts (CAFs) were recruited by the liver metastasis which have a role in tumor progression. The color-coded model of the TME enables its formation to be visualized at the cellular level in vivo, in real-time. This imaging model of the TME should lead to new visual targets in the TME. J. Cell. Biochem. 112: 949-953, 2011. (C) 2010 Wiley-Liss, Inc.