Permuted tRNA genes expressed via a circular RNA intermediate in Cyanidioschyzon merolae
Permuted tRNA genes expressed via a circular RNA intermediate in Cyanidioschyzon merolae
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DOI：
10.1126/science.1145718
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发表时间：
2007-10-19
期刊：
影响因子：
56.9
通讯作者：
Sekine, Yasuhiko
中科院分区：
文献类型：
--
作者：
Soma, Akiko;Onodera, Akinori;Sekine, Yasuhiko
通过对一种红腹蓝腹鱼（Cyanidioschyzonmerolae）的核基因组进行计算机分析，鉴定出11个转运RNA（tRNA）基因，其中tRNA的3'端位于基因组中5'端的上游。我们证实了这些基因表达并产生成熟的氨酰化的tRNA。对这些基因的tRNA加工中间体的分析表明了一种不寻常的加工途径，其中tRNA前体的末端被连接，导致形成特征性的环状RNA中间体，然后在受体茎处加工以产生正确的末端。
A computational analysis of the nuclear genome of a red alga, Cyanidioschyzon merolae, identified 11 transfer RNA (tRNA) genes in which the 3' half of the tRNA lies upstream of the 5' half in the genome. We verified that these genes are expressed and produce mature tRNAs that are aminoacylated. Analysis of tRNA-processing intermediates for these genes indicates an unusual processing pathway in which the termini of the tRNA precursor are ligated, resulting in formation of a characteristic circular RNA intermediate that is then processed at the acceptor stem to generate the correct termini.