Vaccination of cattle with the N terminus of LppQ of Mycoplasma mycoides subsp. mycoides results in type III immune complex disease upon experimental infection.
Vaccination of cattle with the N terminus of LppQ of Mycoplasma mycoides subsp. mycoides results in type III immune complex disease upon experimental infection.
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DOI：
10.1128/iai.00003-15
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发表时间：
2015-05
影响因子：
3.1
通讯作者：
McKeever D
中科院分区：
文献类型：
--
作者：
Mulongo M;Frey J;Smith K;Schnier C;Wesonga H;Naessens J;McKeever D
牛传染性胸膜肺炎（Contagious bovine pleuropneumonia，CBPP）是由丝状支原体肺炎亚种（Mycoplasma mycoides subsp.蕈状。目前针对CBPP的疫苗诱导短暂的免疫力，并可引起严重的疫苗后反应。以前的研究已经确定了M的跨膜脂蛋白Q（LppQ-N′）的N端。蕈状亚种蕈样菌是主要抗原，可能是毒力因子。因此，我们用用弗氏佐剂配制的纯化重组LppQ-N′免疫牛，并用M.蕈状亚种蕈状。免疫动物表现出强烈的血清转化为LppQ，但他们表现出显着增强的攻击后肾小球肾炎相比，安慰剂组（P = 0.021）。肾小球肾炎的特点是提示抗原抗体免疫复合物的发展。接种组和安慰剂组之间的临床体征和大体病理学评分无显著差异。这些发现首次揭示了在攻毒过程中由于抗LppQ抗体而增强疾病的发病机制，并反对将LppQ-N′纳入未来的CBPP亚单位疫苗中。
Contagious bovine pleuropneumonia (CBPP) is a serious respiratory disease of cattle caused by Mycoplasma mycoides subsp. mycoides. Current vaccines against CBPP induce short-lived immunity and can cause severe postvaccine reactions. Previous studies have identified the N terminus of the transmembrane lipoprotein Q (LppQ-N′) of M. mycoides subsp. mycoides as the major antigen and a possible virulence factor. We therefore immunized cattle with purified recombinant LppQ-N′ formulated in Freund's adjuvant and challenged them with M. mycoides subsp. mycoides. Vaccinated animals showed a strong seroconversion to LppQ, but they exhibited significantly enhanced postchallenge glomerulonephritis compared to the placebo group (P = 0.021). Glomerulonephritis was characterized by features that suggested the development of antigen-antibody immune complexes. Clinical signs and gross pathological scores did not significantly differ between vaccinated and placebo groups. These findings reveal for the first time the pathogenesis of enhanced disease as a result of antibodies against LppQ during challenge and also argue against inclusion of LppQ-N′ in a future subunit vaccine for CBPP.