Subcellular localization of rice histone deacetylases in organelles
Subcellular localization of rice histone deacetylases in organelles
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DOI：
10.1016/j.febslet.2009.06.003
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发表时间：
2009-07-07
期刊：
影响因子：
3.5
通讯作者：
Kim, Ju-Kon
中科院分区：
文献类型：
--
作者：
Chung, Pil Joong;Kim, Youn Shic;Kim, Ju-Kon
已知组蛋白脱乙酰酶（HDAC）在细胞核中起作用。在这里，我们报告了三个水稻HDACs，OsSIR 2b，OsHDAC 6和OsHDAC 10的细胞器定位。将35 S：0 sSIR 2b-GFP和35 S：0 sHDAC 10-GFP构建体引入烟草BY2细胞中。绿色的共定位分析。蛋白质和MitoTracker。在转化的BY2细胞中的荧光信号表明OsSIR 2b和OsHDAC 10定位于线粒体中。类似地分析了含有35 S：OsHDAC 6-GFP和35 S：OsHDAC 10-GFP构建体的转基因拟南芥品系，揭示了OsHDAC 6-GFP仅定位于叶绿体中，而OsHDAC 10-GFP定位于线粒体和叶绿体两者中。通过免疫检测证实了0 sHDAC 6-GFP和0 sHDAC 10-GFP在叶绿体中的存在。(C)2009年欧洲生物化学学会联合会。由Elsevier B出版。版权所有© 2016
Histone deacetylases (HDACs) are known to function in the nucleus. Here, we report on the organellar localization of three rice HDACs, OsSIR2b, OsHDAC6, and OsHDAC10. The 35S:OsSIR2b-GFP and 35S:OsHDAC10-GFP constructs were introduced into tobacco BY2 cells. Co-localization analysis of the green. uorescent protein and MitoTracker. uorescent signals in the transformed BY2 cells indicated that OsSIR2b and OsHDAC10 are localized in the mitochondria. Transgenic Arabidopsis lines harboring 35S:OsHDAC6-GFP and 35S: OsHDAC10-GFP constructs were similarly analyzed, revealing that OsHDAC6-GFP is localized exclusively in chloroplasts, whereas OsHDAC10-GFP is localized in both mitochondria and chloroplasts. The presence of OsHDAC6-GFP and OsHDAC10-GFP in chloroplasts was verified by immunodetection. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.