Porphyromonas endodontalis Lipopolysaccharides Induce RANKL by Mouse Osteoblast in a Way Different from That of Escherichia coli Lipopolysaccharide
Porphyromonas endodontalis Lipopolysaccharides Induce RANKL by Mouse Osteoblast in a Way Different from That of Escherichia coli Lipopolysaccharide
复制标题
牙髓卟啉单胞菌脂多糖通过小鼠成骨细胞诱导 RANKL，其方式与大肠杆菌脂多糖不同
DOI：
10.1016/j.joen.2011.08.015
复制
发表时间：
2011-12-01
影响因子：
4.2
通讯作者：
Zhou, Xuedong
中科院分区：
文献类型：
--
作者：
Tang, Yin;Sun, Feifei;Zhou, Xuedong
引言：牙髓卟啉单胞菌脂多糖（LPS）在感染根管和有症状的根尖周炎中已显示出较高的阳性率。它作为一种参与根尖病变的炎性细胞因子的强效刺激物，可能起着不可或缺的作用。核因子-κB受体激活剂配体（RANKL）已被证明是骨重塑的关键调节因子。本研究探讨了牙髓卟啉单胞菌LPS诱导的小鼠成骨细胞中RANKL的产生以及LPS信号传导。
方法：通过蛋白质印迹法和实时聚合酶链反应检测LPS诱导的小鼠成骨细胞MC3T3 - E1中RANKL的产生，并通过使用抗Toll样受体（TLRs）抗体的阻断试验确定TLRs。此外，使用特异性抑制剂分析细胞内信号传导通路。以大肠杆菌LPS作为对照。
结果：抗TLR2和抗TLR4抗体均显著（P＜...此处似乎不完整）
Introduction: Porphyromonas endodontalis lipopolysaccharide (LPS) has been shown to have a high positive rate in infected root canals and symptomatic apical periodontitis. It may play an integral role as a potent stimulator of inflammatory cytokines involved in apical lesions. The receptor activator of nuclear factor-kappa B ligand (RANKL) has been proven to be the key regulator of bone remodeling. This study investigated P endodontalis LPS-induced RANKL production and LPS signaling in mouse osteoblasts. Methods: LPS-induced RANKL production in mouse osteoblast MC3T3-E1 cells was measured by Western blot and real-time polymerase chain reaction, and the Toll-like receptors (TLRs) were determined by the blocking test using anti-TLRs antibodies. In addition, specific inhibitors were used to analyze the intracellular signaling pathways. Escherichia coli LPS was used as the control. Results: Both of the anti-TLR2 and anti-TLR4 antibodies significantly (P