The effect of CPT1B gene on lipid metabolism and its polymorphism analysis in Chinese Simmental cattle
The effect of CPT1B gene on lipid metabolism and its polymorphism analysis in Chinese Simmental cattle
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CPT1B基因对中国西门塔尔牛脂质代谢的影响及其多态性分析
DOI：
10.1080/10495398.2021.1904966
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发表时间：
2021-06-04
影响因子：
3.7
通讯作者：
Fang, Xibi
中科院分区：
文献类型：
--
作者：
He, Wei;Gao, Ming;Fang, Xibi
肉毒碱棕榈酰转移酶1B（Carnitine palmitoyltransferase 1B，CPT 1B）是一个调控家畜脂肪代谢的候选基因，编码脂肪酸β-氧化的限速酶。为探讨CPT 1B基因对牛脂代谢的影响，本研究检测了CPT 1B基因在中国西门塔尔牛中的多态性及其对脂代谢的影响。结果表明，随着CPT 1B基因表达量的增加，牛胎儿成纤维细胞（BFFs）中甘油三酯含量显著增加（p< 0.05），而CPT 1B基因敲除导致CPT 1B表达量降低，甘油三酯含量呈下降趋势。相关分析表明，g.119896238 G > C位点与中国西门塔尔牛背膘厚显著相关（p< 0.05）。GC基因型个体的背膘厚（0.93 ± 0.67 cm）显著大于CC基因型个体（0.84 ± 0.60 cm）。中国西门塔尔牛g.119889302 T > C位点与花生四烯酸含量显著相关（p< 0.05）。CC基因型肉牛背最长肌中花生四烯酸含量（0.054 g/100 g）显著高于其他两种基因型（0.046 g/100 g、0.049 g/100 g）。这些分子标记可有效地用于牛的分子标记辅助选择。
Carnitine palmitoyltransferase 1B (CPT1B) is a candidate gene that regulates livestock animal lipid metabolism and encodes the rate-limiting enzyme in fatty acid β-oxidation. To explore the effect of this gene on lipid metabolism in cattle, this study examined CPT1B gene polymorphism in Chinese Simmental cattle and the effect of CPT1B on lipid metabolism. The results showed that the triglyceride content increased significantly with increasing CPT1B gene expression in bovine fetal fibroblasts (BFFs) (p< 0.05), while CPT1B knockout led to decreased CPT1B expression and a downward trend in triglyceride levels. Correlation analysis showed a significant association between the g.119896238 G > C locus and Chinese Simmental cattle backfat thickness (p< 0.05). Backfat thickness was significantly greater in individuals with the GC genotype (0.93 ± 0.67 cm) than in those with the CC genotype (0.84 ± 0.60 cm). The g.119889302 T > C locus was significantly correlated with arachidonic acid content in Chinese Simmental cattle (p< 0.05). The arachidonic acid content in the longissimus muscle was significantly higher in CC genotype beef cattle (0.054 g/100 g) than in those with the other two genotypes (0.046 g/100 g, 0.049 g/100 g). These molecular markers can be effectively used for marker-assisted selection in cattle breeding.