CYCLIC ADENOSINE-MONOPHOSPHATE IN NERVOUS-SYSTEM OF APLYSIA-CALIFORNICA .1. INCREASED SYNTHESIS IN RESPONSE TO SYNAPTIC STIMULATION
CYCLIC ADENOSINE-MONOPHOSPHATE IN NERVOUS-SYSTEM OF APLYSIA-CALIFORNICA .1. INCREASED SYNTHESIS IN RESPONSE TO SYNAPTIC STIMULATION
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DOI：
10.1085/jgp.60.5.558
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发表时间：
1972-01-01
影响因子：
3.8
通讯作者：
KANDEL, ER
中科院分区：
文献类型：
--
作者：
CEDAR, H;SCHWARTZ, JH;KANDEL, ER
在分离的海兔腹神经节中，预先用腺嘌呤-3‘，5’-单磷酸(CAMP)孵育后，以生理速率(1/秒)电刺激神经后，~3H标记的腺苷-3‘，5’-一磷酸(CAMP)的含量增加一倍。刺激4min后无明显变化。CAMP在15分钟后首次升高；将刺激时间延长至1小时并不增加影响的程度。腺嘌呤-3 H摄取的总放射性中，ATP占50%，cAMP约占0.1%。在刺激过程中，三磷酸腺苷(ATP)的总量和比放射性都没有变化。因此，在刺激后cAMP中发现的放射性增加代表其合成速度的增加。在刺激过程中，cAMP-3H在神经或已识别神经元(R2)的细胞体中的形成没有改变。此外，R2神经节和胞体内cAMP总量未见变化。这种增加很可能是由突触活动而不是动作电位引起的。它是通过提高镁的浓度来阻断的，镁也可以在不损害冲动传导的情况下阻止突触活动。此外，哇巴因和谷氨酸诱发的冲动活动不会增加cAMP的形成。
In the isolated abdominal ganglion ofAplysia, previously incubated in adenine-3H, the amount of3H-labeled adenosine-3',5' monophosphate (cAMP) doubled after electrical stimulation of nerves at a physiological rate (1/sec). No change was detected after 4 min of stimulation. An increase in cAMP was first seen after 15 min; lengthening the period of stimulation to 1 hr did not increase the extent of the effect. ATP contained 50% of the total radioactivity taken up from adenine-3H, cAMP about 0.1%. During stimulation both the total amount and the specific radioactivity of adenosine triphosphate (ATP) did not change. Thus, the increased amount of radioactivity found in cAMP after stimulation represented an increase in its rate of synthesis. During stimulation formation of cAMP-3H was not altered in nerves or in the cell body of an identified neuron (R2). In addition, no changes were detected in the total amounts of cAMP in the ganglion and in the cell body of R2. It seems likely that the increase was initiated by synaptic activity rather than by action potentials. It was blocked by elevating the concentration of Mg, which also blocks synaptic activity without impairing conduction of impulses. Moreover, impulse activity induced by ouabain and glutamate did not result in increased formation of cAMP.