Isolation and identification of the most efficient plant growth-promoting bacteria associated with canola (Brassica napus)

Isolation and identification of the most efficient plant growth-promoting bacteria associated with canola (Brassica napus)
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DOI:
10.1007/s003740000305
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发表时间:
2001-02-01
影响因子:
6.5
通讯作者:
Cleyet-Marel, JC
Cleyet-Marel, JC
中科院分区:
农林科学1区
文献类型:
--
作者:
Bertrand, H;Nalin, R;Cleyet-Marel, JC

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从油菜根际和根际土壤中分离出13株能在氮素缺乏的培养条件下生长的革兰氏阴性细菌,并通过16 SrDNA序列分析鉴定。这些细菌中的8个诱导了根干重的显着增加,范围从11%至52%。基于16 S rDNA序列的系统发育定位表明,至少有4个属,假单胞菌属(Pseudomonas)、Vario-vorax、土壤杆菌属(Agrobacterium)和叶杆菌属(Phyllobacterium)。
Thirteen of the most abundant Gram-negative bacteria which are able to grow in N-depleted culture conditions were isolated from the rhizoplane and endorhizosphere of canola (Brassica napus) and identified by 16S rDNA sequence analysis. Eight of these bacteria induced a significant increase in root dry weight ranging from 11 to 52%. Phylogenetic positioning based on 16S rDNA sequences indicated that at least four genera are represented, Pseudomonas, Vario-vorax, Agrobacterium and Phyllobacterium, The most important direct plant growth-promoting-rhizobacteria effect was found with both isolates belonging to the Phyllobacterium.