PARAMETERS CONTROLLING TRANSCRIPTIONAL ACTIVATION DURING EARLY DROSOPHILA DEVELOPMENT
PARAMETERS CONTROLLING TRANSCRIPTIONAL ACTIVATION DURING EARLY DROSOPHILA DEVELOPMENT
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DOI：
10.1016/0092-8674(86)90009-7
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发表时间：
1986-03-28
期刊：
影响因子：
64.5
通讯作者：
SCHUBIGER, G
中科院分区：
文献类型：
--
作者：
EDGAR, BA;SCHUBIGER, G
我们研究了转录过程中的第一个14有丝分裂周期的果蝇发展，通过凝胶电泳的RNA脉冲标记在体内。rRNA、tRNA、5S RNA、snRNA、poly（A）+RNA和组蛋白mRNA的合成在第11或12周期期间首次可检测到。组蛋白基因在S期转录，并在第12周期达到最大活化，而非组蛋白基因仅在G2期转录，并在第14周期晚期达到最大活化。周期14的高转录活性特征可以通过用放线菌酮延长间期早在周期10（但不是在周期10之前）而被早熟诱导。我们的结论是，所有类别的基因成为有能力在第10个周期的激活，随后的激活差异抑制核分裂相关的功能。
We studied transcription during the first 14 mitotic cycles of Drosophila development, by gel electrophoresis of RNA pulse-labeled in vivo. Synthesis of rRNA, tRNAs, 5S RNAs, snRNAs, poly(A)+ RNAs, and histone mRNAs is first detectable during cycle 11 or 12. Histone genes are transcribed during S phases, and reach maximal activation in cycle 12, whereas nonhistone genes are transcribed only in G2 periods, and reach maximal activation during late cycle 14. The high transcriptional activity characteristic of cycle 14 can be precociously induced by extending interphase with cycloheximide as early as, but not before, cycle 10. We conclude that all classes of genes become competent for activation during cycle 10, and that subsequent activation is differentially suppressed by functions associated with nuclear division.