Label-free cell nuclear imaging by Grüneisen relaxation photoacoustic microscopy.
Label-free cell nuclear imaging by Grüneisen relaxation photoacoustic microscopy.
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DOI：
10.1364/ol.43.000947
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发表时间：
2018-02-15
期刊：
影响因子：
3.6
通讯作者：
Wang LV
中科院分区：
文献类型：
--
作者：
Liu X;Wong TTW;Shi J;Ma J;Yang Q;Wang LV
光声显微镜（PAM）与紫外线（UV）激光照明最近已被证明是一个很有前途的工具，提供快速，无标记，和多层的人类乳腺组织的组织学成像。到目前为止，轴向分辨率已确定超声。为了实现光学定义的轴向分辨率，我们利用Grüneisen弛豫（GR）效应。通过对小鼠脑切片进行成像，我们发现GRUV-PAM揭示了有关三维细胞核分布和内部结构的详细信息，这些信息是癌症的重要诊断特征。由于非线性效应，GRUV-PAM还在细胞核的图像中提供更好的对比度。
Photoacoustic microscopy (PAM) with ultraviolet (UV) laser illumination has recently been demonstrated as a promising tool that provides fast, label-free, and multilayered histologic imaging of human breast tissue. Thus far, the axial resolution has been determined ultrasonically. To enable optically defined axial resolution, we exploit the Grüneisen relaxation (GR) effect. By imaging mouse brain slices, we show that GRUV-PAM reveals detailed information about three-dimensional cell nuclear distributions and internal structures, which are important diagnostic features for cancers. Due to the nonlinear effect, GRUV-PAM also provides better contrast in images of cell nuclei.