Adaptations of Pseudomonas aeruginosa to the cystic fibrosis lung environment can include deregulation of zwf, encoding glucose-6-phosphate dehydrogenase.
Adaptations of Pseudomonas aeruginosa to the cystic fibrosis lung environment can include deregulation of zwf, encoding glucose-6-phosphate dehydrogenase.
复制标题
铜绿假单胞菌对囊性纤维化肺环境的适应可包括编码葡萄糖-6-磷酸脱氢酶的 zwf 的失调。
DOI：
10.1128/jb.187.22.7561-7568.2005
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发表时间：
2005
影响因子：
3.2
通讯作者：
Ohman,DennisE
中科院分区：
文献类型：
--
作者：
Silo-Suh,Laura;Suh,Sang-Jin;Phibbs,PaulV;Ohman,DennisE
Cystic fibrosis (CF) patients are highly susceptible to chronic pulmonary disease caused by mucoidPseudomonas aeruginosastrains that overproduce the exopolysaccharide alginate. We showed here that a mutation inzwf, encoding glucose-6-phosphate dehydrogenase (G6PDH), leads to a ∼90% reduction in alginate production in the mucoid, CF isolate,P. aeruginosaFRD1. The main regulator of alginate, sigma-22 encoded byalgT(algU), plays a small but demonstrable role in the induction ofzwfexpression inP. aeruginosa. However, G6PDH activity andzwfexpression were higher in FRD1 strains than in PAO1 strains. In PAO1,zwfexpression and G6PDH activity are known to be subject to catabolite repression by succinate. In contrast, FRD1zwfexpression and G6PDH activity were shown to be refractory to such catabolite repression. This was apparently not due to a defect in the catabolite repression control (Crc) protein. Such relaxed control ofzwfwas found to be common among several examined CF isolates but was not seen in other strains of clinical and environmental origin. Two sets of clonal isolates from individual CF patient indicated that the residentP. aeruginosastrain underwent an adaptive change that deregulatedzwfexpression. We hypothesized that high-level, unregulated G6PDH activity provided a survival advantage toP. aeruginosawithin the lung environment. Interestingly,zwfexpression inP. aeruginosawas shown to be required for its resistance to human sputum. This study illustrates that adaptation to the CF pulmonary environment byP. aeruginosacan include altered regulation of basic metabolic activities, including carbon catabolism.
