3D Cell Culture in Alginate Hydrogels.
3D Cell Culture in Alginate Hydrogels.
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DOI：
10.3390/microarrays4020133
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发表时间：
2015-03-24
期刊：
影响因子：
--
通讯作者：
Dornish M
中科院分区：
文献类型：
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作者：
Andersen T;Auk-Emblem P;Dornish M
这篇综述汇编了有关藻酸盐，特别是藻酸盐水凝胶在3D培养细胞中的使用的信息。藻酸盐化学结构和功能的知识被证明是设计细胞培养用藻酸盐基质的重要参数。凝胶弹性和水凝胶稳定性受所用海藻酸盐的类型、其浓度、凝胶技术(离子或共价)的选择以及作为凝胶诱导离子的二价阳离子的选择的影响。使用多肽偶联的海藻酸盐可以控制细胞与基质的相互作用。藻酸盐的凝胶化伴随着细胞的固定化可以有多种形式。自20世纪80年代以来，液滴或珠子就被用来固定细胞。大孔支架等较新的基质现在正在进入新的3D细胞培养产品市场。最后，延迟凝胶、注射、海藻酸盐系统在将体外细胞培养转化为体内组织工程应用方面显示出有效性。藻酸盐在3D细胞培养方面有历史和未来。在历史上，细胞被包裹在含有钙的海藻酸盐液滴中，用于人工器官的发育。现在，几种基于海藻酸盐的商业产品正被用作3D细胞培养系统，这也展示了替换或再生组织的可能性。
This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of alginate used, its concentration, the choice of gelation technique (ionic or covalent), and divalent cation chosen as the gel inducing ion. The use of peptide-coupled alginate can control cell–matrix interactions. Gelation of alginate with concomitant immobilization of cells can take various forms. Droplets or beads have been utilized since the 1980s for immobilizing cells. Newer matrices such as macroporous scaffolds are now entering the 3D cell culture product market. Finally, delayed gelling, injectable, alginate systems show utility in the translation of in vitro cell culture to in vivo tissue engineering applications. Alginate has a history and a future in 3D cell culture. Historically, cells were encapsulated in alginate droplets cross-linked with calcium for the development of artificial organs. Now, several commercial products based on alginate are being used as 3D cell culture systems that also demonstrate the possibility of replacing or regenerating tissue.