Gene Expression of ANP, BNP and ET-1 in the Heart of Rats during Pulmonary Embolism
Gene Expression of ANP, BNP and ET-1 in the Heart of Rats during Pulmonary Embolism
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DOI：
10.1371/journal.pone.0011111
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发表时间：
2010-06-14
期刊：
影响因子：
3.7
通讯作者：
Kjaer, Andreas
中科院分区：
文献类型：
--
作者：
Gutte, Henrik;Oxbol, Jytte;Kjaer, Andreas
目的：心房钠尿肽（ANP）、脑钠尿肽（BNP）和内皮素-1（ET-1）可能反映肺栓塞（PE）患者右心室功能障碍（RVD）的严重程度。 PE后BNP、ANP和ET-1表达和分泌的确切性质和来源尚未研究。方法和结果：注射聚苯乙烯微粒以诱导大鼠PE。通过实时定量聚合酶链式反应 (QPCR) 测定 4 个心腔中 BNP、ANP 和 ET-1 的基因表达。测量血浆中 ANP、BNP、ET-1 和心肌肌钙蛋白 I (TNI) 的血浆水平。 PE 剂量依赖性地增加右心室 (RV) 中 ANP 和 BNP 的基因表达，并增加右心房 (RA) 中 ANP 的基因表达。相反，PE 剂量依赖性地降低左心室 (LV) 和左心房 (LA) 中的 BNP 基因表达。血浆BNP、TNI和ET-1水平随PE程度呈剂量依赖性升高。结论：我们发现PE程度与心室ANP和BNP基因表达密切相关，且右心室选择性升高。目前的数据支持利钠肽作为 PE 中 RVD 有价值的生物标志物的想法。
Aims: Atrial natriuretic petide (ANP), brain natriuretic peptide (BNP) and endothelin-1 (ET-1) may reflect the severity of right ventricular dysfunction (RVD) in patients with pulmonary embolism (PE). The exact nature and source of BNP, ANP and ET-1 expression and secretion following PE has not previously been studied.Methods and Results: Polystyrene microparticles were injected to induce PE in rats. Gene expression of BNP, ANP and ET-1 were determined in the 4 cardiac chambers by quantitative real time polymerase chain reaction (QPCR). Plasma levels of ANP, BNP, ET-1 and cardiac troponin I (TNI) were measured in plasma. PE dose-dependently increased gene expression of ANP and BNP in the right ventricle (RV) and increased gene expression of ANP in the right atrium (RA). In contrast PE dose-dependently decreased BNP gene expression in both the left ventricle (LV) and the left atrium (LA). Plasma levels of BNP, TNI and ET-1 levels dose-dependently increased with the degree of PE.Conclusion: We found a close correlation between PE degree and gene-expression of ANP, and BNP in the cardiac chambers with a selective increase in the right chambers of the heart. The present data supports the idea of natriuretic peptides as valuable biomarkers of RVD in PE.