Filamentous marine fungi as producers of O-glycosylhydrolases:: β-1,3-glucanase from Chaetomium indicum
Filamentous marine fungi as producers of O-glycosylhydrolases:: β-1,3-glucanase from Chaetomium indicum
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DOI：
10.1007/s10126-002-0070-2
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发表时间：
2003-07-01
影响因子：
3
通讯作者：
Zvyagintseva, TN
中科院分区：
文献类型：
--
作者：
Burtseva, YV;Verigina, NS;Zvyagintseva, TN
研究了从海洋环境中分离的42种真菌菌株产胞外酶的能力。这些菌株的培养物显示含有一系列随生境而变化的糖苷酶（β-葡糖苷酶、N-乙酰-β-氨基葡糖苷酶、β-半乳糖苷酶、α-甘露糖苷酶）和葡聚糖酶（1，3-β-葡聚糖酶、淀粉酶）。活性水平取决于真菌的种类。已经分离出几种有前途的菌株，其能够产生用于裂解含碳水化合物的单个酶和一组酶。确定了印度毛壳菌生长和β-1，3-葡聚糖酶生物合成的最佳条件。采用离子交换层析、超滤和凝胶过滤分离β-1，3-葡聚糖酶。显示存在2种酶形式;两种形式均为外切-β-1，3-葡聚糖酶。
Ninety fungal strains (42 species) isolated from marine habitats were studied for their ability to produce extracellular enzymes. Cultural filtrates of these strains were shown to contain a series of glycosidases (beta-glucosidases, N-acetyl-beta-glucosaminidases, beta-galactosidases alpha-mannosidases) and glucanases (1,3-beta-glucanases, amylases) which varied with habitat. The level of activity depended on the species of fungi. Several promising strains capable of producing both individual enzymes and a set of enzymes for splitting carbohydrate-containing compound have been isolated. Optimal conditions for growth of Chaetomium indicum and for biosynthesis of beta-1,3-glucanase were determined. beta-1,3-Glucanase was isolated using ion-exchange chromatography, ultrafiltration, and gel filtration. The presence of 2 enzyme forms was shown; both forms were exo-beta-1,3-glucanases.