A rat model of saliva secretory immunoglobulin: A suppression caused by intense exercise
A rat model of saliva secretory immunoglobulin: A suppression caused by intense exercise
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DOI：
10.1111/j.1600-0838.2007.00642.x
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发表时间：
2008-06-01
影响因子：
4.1
通讯作者：
Kono, I.
中科院分区：
文献类型：
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作者：
Kimura, F.;Aizawa, K.;Kono, I.
本研究旨在建立一种有效的大强度运动诱导免疫抑制大鼠模型。将大鼠分为三组。在休息（Rest）组中，连续4天从休息大鼠收集唾液。在运动（Ex）组中，大鼠在跑步机上跑步直到力竭（运动时间：60.0 +/-3.7 min），并且在运动前后收集它们的唾液;运动后去除唾液腺。在对照组（Con）中，也进行唾液收集和腺体切除，但大鼠不运动。测量唾液中分泌性免疫球蛋白A（SIgA）浓度和腺体中多聚免疫球蛋白受体（pIgR）mRNA表达。休息组4天内SIgA浓度无显著变化。在Ex组中，SIgA浓度在运动后与运动前相比显著降低，而Con组中无显著变化。运动后Ex组pIgR mRNA的表达显著低于Con组。我们的唾液收集程序似乎是合适的，运动诱导的SIgA抑制可能是由pIgR mRNA表达下降引起的。我们建议在未来的研究中使用这种可重复和可靠的运动诱导的SIgA抑制大鼠模型。
We aimed to develop a valid model of immunosuppression induced by intense exercise in rats. Rats were divided into three groups. In the rest (Rest) group, saliva was collected from resting rats on 4 consecutive days. In the exercise (Ex) group, rats ran on a treadmill untill exhaustion (exercise time: 60.0 +/- 3.7 min), and their saliva was collected before and after exercise; the salivary glands were removed after exercise. In the control (Con) group, saliva collection and gland removal were also performed, but the rats did not exercise. Secretory immunoglobulin A (SIgA) concentrations in saliva and polymeric immunoglobulin receptor (pIgR) mRNA expression in the glands were measured. There was no significant change in SIgA concentration in the Rest group over 4 days. In the Ex group, SIgA concentration decreased significantly after exercise compared with before, whereas there was no significant change in the Con group. The expression of pIgR mRNA was significantly lower in the Ex group post-exercise than in the Con group. Our procedure for saliva collection appeared suitable, and the exercise-induced SIgA suppression was probably caused by a decline in pIgR mRNA expression. We propose to use this reproducible and reliable rat model of exercise-induced SIgA suppression in future studies.